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CD107a antibody | 4E9/11

Product Code Applications Pack Size List Price Your Price Qty
MCA2315GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IP P * WB 0.1 mg loader
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loader

Mouse anti Pig CD107a, clone 4E9/11 recognizes porcine CD107a, a cell surface antigen, also known as lysosomal-associated membrane protein-1 or LAMP-1.

CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/25 1/50
Immunohistology - Frozen
Immunohistology - Paraffin 2
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
Histology Positive Control Tissue
Porcine spleen

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References for CD107a antibody

  1. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
    Tissue Antigens. 49 (4): 403-13.
  2. Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
    Vet Immunol Immunopathol. 89 (1-2): 91-8.
  3. Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
    Vet Immunol Immunopathol. 94 (1-2): 77-81.
  4. Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
    Arch Virol. 148 (12): 2307-23.
  5. Toka, F.N. et al. (2009) Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus.
    Clin Vaccine Immunol. 16: 1738-49.
  6. Toka, F.N. et al. (2009) Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells.
    J Interferon Cytokine Res. 29 (3): 179-92.
  7. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  8. Cruz, J.L. et al. (2013) Alphacoronavirus Protein 7 Modulates Host Innate Immune Response
    J Virol. 87: 9754-67.
  9. View The Latest Product References
  10. Bullers, S.J. et al. (2014) The human tissue-biomaterial interface: a role for PPARγ-dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype.
    Tissue Eng Part A. 20: 2390-401.
  11. van Hout, G.P. et al. (2015) Invasive surgery reduces infarct size and preserves cardiac function in a porcine model of myocardial infarction.
    J Cell Mol Med. 19 (11): 2655-63.
  12. Talker, S.C. et al. (2015) Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
    Vet Res. 46: 52.
  13. Bongoni, A.K. et al. (2015) Porcine extrahepatic vascular endothelial asialoglycoprotein receptor 1 mediates xenogeneic platelet phagocytosis in vitro and in human-to-pig ex vivo xenoperfusion.
    Transplantation. 99 (4): 693-701.
  14. Dash, R. et al. (2018) Dose-Dependent Cardioprotection of Moderate (32°C) Versus Mild (35°C) Therapeutic Hypothermia in Porcine Acute Myocardial Infarction.
    JACC Cardiovasc Interv. 11 (2): 195-205.
  15. Nishimura, Y. et al. (2023) Application of polyglycolic acid sheets and basic fibroblast growth factor to prevent esophageal stricture after endoscopic submucosal dissection in pigs.
    J Gastroenterol. 58 (11): 1094-104.
  16. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16): 2103.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Synonyms
LAMP-1

MCA2315GA

149602 1610 163391

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