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B Cell Subset antibody | BB6-10A10

Mouse anti Pig B Cell Subset:FITC

Product Type
Monoclonal Antibody
Clone
BB6-10A10
Isotype
IgM
Specificity
B Cell Subset

Product Code Applications Pack Size List Price Your Price Qty
MCA6099F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig B cell subset antibody, clone BB6-10A10, appears to recognize an immature pig B cell population. Clone BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood, 22-23% in mesenteric lymph nodes, 79-87% in ileal Peyer’s patches, 6-17 in spleen, and 0% in thymus (Boersma et al. 2001).

Target Species
Pig
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgM prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgM concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Pig CD45RA:RPE MCA1751PE F 100 Tests
List Price Your Price
Description Mouse anti Pig CD45RA:RPE

References for B Cell Subset antibody

  1. Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
    Vet Immunol Immunopathol. 80 (1-2): 63-78.

MCA6099F

151598 163969

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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