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Phosphotyrosine antibody | PY20

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Mouse anti Phosphotyrosine:RPE

Product Type
Monoclonal Antibody
Clone
PY20
Isotype
IgG2b
Specificity
Phosphotyrosine

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Product Code Applications Pack Size List Price Your Price Qty
140009
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Mouse anti Phosphotyrosine, Clone PY20, is specific for phosphotyrosine. Phosphorylation of tyrosine residues is considered a key stem in signal transduction and regulation of enzymatic activity.

Target Species
Chemical
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Approx. Protein Concentrations
0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Phosphotyrosine antibody

  1. Vendel,. A.C. et al. (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
    J Immunol. 182: 1509-17.
  2. van der Wel, T. et al. (2020) Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis.
    Nat Commun. 11 (1): 3216.

RRID
AB_616874
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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