Panitumumab antibody | AbD23897_hIgG1

Human anti Panitumumab:HRP

Product Type
Monoclonal Antibody
Clone
AbD23897_hIgG1
Isotype
IgG1
Specificity
Panitumumab

Product Code Applications Pack Size List Price Your Price Qty
HCA265P
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human anti panitumumab, clone AbD23897_hIgG1, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody drug panitumumab and inhibits it binding to its target, epidermal growth factor receptor (EGFR). The antibody can be used to measure the levels of panitumumab and biosimilar products in bioanalytical assays.

Clone AbD23897_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD23894 (HCA264) in monovalent Fab format, as the capture antibody.

Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signaling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer.

View a summary of all anti panitumumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Panitumumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD = 0.3 nM by real time, label free molecular interaction analysis on immobilized panitumumab.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection antibody together with HCA264 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Panitumumab HCA264 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Panitumumab
Human anti Panitumumab HCA266 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Panitumumab

References for Panitumumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118A1

Synonyms
VECTIBIX®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"