Browsing Modes for Business Users

Nivolumab antibody | AbD30255

Human anti Nivolumab

Product Type
Monoclonal Antibody
Clone
AbD30255
Isotype
HuCAL Fab monovalent
Specificity
Nivolumab

Product Code Applications Pack Size List Price Your Price Qty
HCA299
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Nivolumab Antibody, clone AbD30255 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug nivolumab. The antibody does not recognize recombinant human programmed cell death 1 (PD-1) or nivolumab in complex with recombinant human PD-1 and can be used to measure free nivolumab levels in serum from patients.

A pair of anti-nivolumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an HRP conjugated Anti-Nivolumab Antibody in full immunoglobulin format, clone AbD30258_hIgG1 (HCA301) as the detection antibody.

Nivolumab (Opdivo) is a fully human antibody (IgG4/kappa), with the heavy chain mutation S228P (IgG4-Pro). It is used as a first line treatment for inoperable or metastatic melanoma in combination with ipilimumab if the cancer does not have a mutation in BRAF. It is also approved for treatment of advanced non-small cell lung cancer, advanced renal cell carcinoma, classical Hodgkin's lymphoma and advanced head and neck squamous cell carcinoma. Nivolumab is a checkpoint inhibitor and acts as an immuno-modulator by blocking ligand activation of PD-1 receptor on T cells, thereby activating T cells to attack the cancer. Nivolumab blocks PD-1 interaction with PD-1 ligand (PD-L1) and PD-1 ligand 2 (PD-L2).

View a summary of all anti-nivolumab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Nivolumab
Affinity
The intrinsic affinity of the monovalent form of Human anti Nivolumab antibody, clone AbD30255 is KD = 0.5 nM as measured by real time, label free molecular interaction analysis on immobilized Nivolumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Opdivo is a trademark of Bristol-Myers Squibb Company.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Nivolumab antibody, clone AbD30255 can be used in a direct or indirect ELISA system or as capture antibody for nivolumab in a bridging ELISA together with HCA301 (AbD30258_hIgG1) as the detection reagent.
Protocol: PK bridging ELISA to measure free drug .

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat anti DYKDDDDK Tag MCA4764 E F IF IP P WB 0.2 mg loader
List Price Your Price
loader
Description Rat anti DYKDDDDK Tag
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
HuCAL Fab-FH Negative Control HCA045 E 0.1 mg loader
>
List Price Your Price
loader
Description HuCAL Fab-FH Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Nivolumab HCA301 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Nivolumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA299

151415 160510 162791 166863

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with NIVOLUMAB specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note