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TIM-3 antibody | RMT3-23

Product Code Applications Pack Size List Price Your Price Qty
MCA5790GA
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SDS Safety Datasheet SDS
C F IF P 0.1 mg loader
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loader

Rat anti Mouse Tim-3 antibody, clone RMT3-23 recognizes mouse T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells.

Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain.

Rat anti Mouse Tim-3 antibody, clone RMT3-23 significantly inhibits the efficient phagocytosis of apoptotic cells by peritoneal exudate Mac1+ cells ( Nakayama et al. 2009).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Tim-3-Ig, consisting of amino acids 1-191 of mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for TIM-3 antibody

  1. Oikawa, T. et al. (2006) Preferential involvement of Tim-3 in the regulation of hepatic CD8+ T cells in murine acute graft-versus-host disease.
    J Immunol. 177 (7): 4281-7.
  2. Nakayama, M. et al. (2009) Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation.
    Blood. 113 (16): 3821-30.

Further Reading

  1. Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
    Immunol Rev. 235 (1): 172-89.

Synonyms
T Cell Immunoglobulin Mucin 3
UniProt
Q8VIM0
Entrez Gene
Havcr2
GO Terms
GO:0016021 integral to membrane

MCA5790GA

151463 1607

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