TER-119/Erythroid Cells antibody | TER-119
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Rat anti Mouse TER-119/Erythroid Cells:FITC
- Product Type
- Monoclonal Antibody
- Clone
- TER-119
- Isotype
- IgG2b
- Specificity
- TER-119/Erythroid Cells
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| Rat anti Mouse TER-119/erythroid cells antibody, clone TER-119 recognizes TER-119 also known as Ly-76. The TER-119 clone detects a 52 kDa protein that is associated with cell surface expressed glycophorin A. TER-119 is an erythroid-specific antigen expressed from the early proerythroblast through to the mature erythrocyte stage. This antigen is also expressed on 20-25% of bone marrow cells and 2-3% of spleen cells but not on thymocytes, lymph node cells or erythroid colony-forming cells (Kina et al. 2000). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Day 14 fetal liver cells from a C57BL/6 mouse
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
Further Reading
-
Kina, T. et al. (2000) The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage.
Br J Haematol. 109 (2): 280-7.
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