TER-119/Erythroid Cells antibody | TER-119

Rat anti Mouse TER-119/Erythroid Cells:FITC

Product Type
Monoclonal Antibody
Clone
TER-119
Isotype
IgG2b
Specificity
TER-119/Erythroid Cells

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MCA6044F
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Rat anti Mouse TER-119/erythroid cells antibody, clone TER-119 recognizes TER-119 also known as Ly-76. The TER-119 clone detects a 52 kDa protein that is associated with cell surface expressed glycophorin A. TER-119 is an erythroid-specific antigen expressed from the early proerythroblast through to the mature erythrocyte stage. This antigen is also expressed on 20-25% of bone marrow cells and 2-3% of spleen cells but not on thymocytes, lymph node cells or erythroid colony-forming cells (Kina et al. 2000).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Day 14 fetal liver cells from a C57BL/6 mouse
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Further Reading

  1. Kina, T. et al. (2000) The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage.
    Br J Haematol. 109 (2): 280-7.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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