Padi2 antibody | AbD02121

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Human anti Mouse Padi2

Product Type
Monoclonal Antibody
Clone
AbD02121
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA011 C E WB 0.1 mg
Human anti mouse Padi2 antibody recognizes Peptidylarginine deiminase II also known as Padi2 or mKIAA0994.

Human anti mouse Padi2 may bind to protein-arginine deiminase type II of others species, due to a high sequence identity in the antigen region. The cross-species identity is: human 98%, rat 98%, chimpanzee 97%, dog 97%, chicken 87%.

The mKIAA0994 protein catalyzes the deimination of arginine residues of proteins. Protein L-arginine reacts to protein L-citrulline and ammonia. The enzyme requires calcium as cofactor. mKIAA0994 is expressed in various tissues including muscle, uterus, spinal cord, salivary gland and pancreas. It is expressed during the estrous cycle. During diestrus and proestrus cycle, the expression level drops eight fold. The protein belongs to the protein-arginine deiminase family.

Product Details

Target Species
Mouse
Product Form
A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Reconstitution
Reconstitute with 0.1 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
A fusion protein of a domain of murine mKIAA0994 (amino acid residues 203-389, MW 21 kDa) expressed in E. coli.
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml following reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
6 months from date of reconstitution

More Information

UniProt
Q08642 Related reagents
Entrez Gene
Padi2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0004668 protein-arginine deiminase activity
GO:0005737 cytoplasm
GO:0018101 peptidyl-citrulline biosynthetic process from peptidyl-arginine
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany, in collaboration with the Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818 JAPAN.

His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Padi2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.0 ug/ml
Immunohistology - Frozen 3.6 ug/ml
Western Blotting 2.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Goat anti Human IgG F(ab')2:Alk. Phos. 0500-0100 0.5 ml E WB
Mouse anti c-Myc MCA2200 1 mg C E F* P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Product Specific References

References for Padi2 antibody

  1. Ohara, R. et al. (2006) Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology.
    Proteomics. 6 (9): 2638-46.

Further Reading

  1. Tsuchida, M. et al. (1993) cDNA nucleotide sequence and primary structure of mouse uterine peptidylarginine deiminase. Detection of a 3'-untranslated nucleotide sequence common to the mRNA of transiently expressed genes and rapid turnover of this enzyme's mRNA in the estrous cycle.
    Eur J Biochem. 215 (3): 677-85.
  2. Strausberg, R.L. et al. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proc Natl Acad Sci U S A. 99 (26): 16899-903.
  3. Terakawa, H. et al. (1991) Three types of mouse peptidylarginine deiminase: characterization and tissue distribution.
    J Biochem. 110 (4): 661-6.
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