NKG2A/C/E antibody | 20d5

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Rat anti Mouse NKG2A/C/E:Low Endotoxin

Product Type
Monoclonal Antibody
Clone
20d5
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2287EL
Datasheet
F FN
SDS
0.5 mg loader

Rat anti Mouse NKG2A/C/E antibody, clone 20d5 recognizes murine NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells.
The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse (Colmenero et al. 2007).
Rat anti Mouse NKG2A/C/E, clone 20d5 is reported to block ligand binding to the receptor (Vance et al. 1999)

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
CHO transfected cells expressing the B6 allele of NKG2A.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Endotoxin Level
< 0.01 EU/ug
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of NKG2A/C/E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL F 0.5 mg loader

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for NKG2A/C/E antibody

  1. Vance, R.E. et al. (1999) Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells.
    J Exp Med. 190 (12): 1801-12.
  2. Vance, R.E. et al. (2002) Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation.
    Proc Natl Acad Sci U S A. 99 (2): 868-73.
  3. Kawamura, H. et al. (2005) Amelioration of acute graft-versus-host disease by NKG2A engagement on donor T cells.
    Eur J Immunol. 35 (8): 2358-66.
  4. Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.
    Cancer Immunol Res. 1 (4): 256-68.
  5. Colmenero, P. et al. (2007) Qa-1(b)-dependent modulation of dendritic cell and NK cell cross-talk in vivo.
    J Immunol. 179: 4608-15.

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