Rat anti Mouse NKG2A/C/E antibody, clone 20d5
recognizes murine NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells.
The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse (Colmenero et al. 2007
Rat anti Mouse NKG2A/C/E, clone 20d5 is reported to block ligand binding to the receptor (Vance et al. 1999
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- CHO transfected cells expressing the B6 allele of NKG2A.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Endotoxin Level
- < 0.01 EU/ug
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NKG2A/C/E antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for NKG2A/C/E antibody
Vance, R.E. et al. (1999) Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells.
J Exp Med. 190 (12): 1801-12.
Vance, R.E. et al. (2002) Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation.
Proc Natl Acad Sci U S A. 99 (2): 868-73.
Kawamura, H. et al. (2005) Amelioration of acute graft-versus-host disease by NKG2A engagement on donor T cells.
Eur J Immunol. 35 (8): 2358-66.
Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.
Cancer Immunol Res. 1 (4): 256-68.
Colmenero, P. et al. (2007) Qa-1(b)-dependent modulation of dendritic cell and NK cell cross-talk in vivo.
J Immunol. 179: 4608-15.
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