NC1.1 antibody | IC4

100% Secure

Mouse anti Mouse NC1.1:Preservative Free

Product Type
Monoclonal Antibody
Clone
IC4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1763XZ F FN IP WB 1 mg
Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes an alloantigen designated NC1.1 expressed by murine natural cytotoxic cells. (NC1.1 is a 45kDa cell surface antigen).

Natural cytotoxic cells represent a small subset of cells with cytotoxic activity against certain tumor cells in functional assays. Mouse anti Mouse NC1.1 antibody, clone IC4 has been shown to block substantially NC cells activity in vivo and in vitro.

Mouse anti Mouse NC1.1 antibody, clone IC4 recognizes the NC1.1 alloantigen in CBA, C57BL/6, BALB/c and NZB mice, but not in CE or DBA/2 mice.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Spleen cells from CBA mice
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized CE mice were fused with cells of the mouse NS-I myeloma cell line.

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of NC1.1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/40
Functional Assays
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested wokring dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for NC1.1 antibody

  1. Smart, Y.C. et al. (1990) Production of a monoclonal allo-antibody to murine natural cytotoxic cells.
    Immunol. Cell Biol. 68: 277-284.
  2. Smart, Y.C. et al. (1990) Natural Cytotoxic cells and tumour surveillance in vivo.
    Eur. J. Cancer 26: 863-864.
  3. Smart, Y.C. et al. (1992) Correlation of growth of tumours in NC-cell depleted mice with NC- and NK-cell mediated lysis in vitro.
    Int. J. Cancer 50: 817-821.
  4. Brien, J.H. et al. (1994) Phenotype and morphology of murine NC1.1+ natural cytotoxic cells.
    Immunol. Cell. Biol. 72: 161-168.
  5. Holmgreen, S.P. et al. (1997) Phosphorylation of the NC-1.1 receptor and regulation of natural cytotoxicity by Protein kinase C and cyclic GMP-dependent protein kinase.
    J. Immunol. 158: 2035-2041.