MHC Class II antibody | IBL-3/5
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Rat anti Mouse MHC Class II antibody, clone IBL-3/5 recognizes a monomorphic epitope on murine MHC Class II molecules., The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
Rat anti Mouse MHC Class II antibody, clone IBL-3/5 induces homotypic clustering of B cells in vitro (Balogh 1995 In 9th International Congress of Immunology, Abstract book p. 72. No. 422).
MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
Rat anti Mouse MHC Class II antibody, clone IBL-3/5 induces homotypic clustering of B cells in vitro (Balogh 1995 In 9th International Congress of Immunology, Abstract book p. 72. No. 422).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- in vivo stimulated, purified mouse B cells.
- Fusion Partners
- Spleen cells from an immunized Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | ||
| Immunohistology - Frozen 1 | Neat | ||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Western Blotting |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 105 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgM:Biotin | 302008 | C E IB | 1 mg |
|
|
| Goat anti Rat IgM:RPE | 302009 | F | 0.5 mg |
|
|
| Goat anti Rat IgM:HRP | STAR116P | E WB | 0.5 mg |
|
Product Specific References
References for MHC Class II antibody
-
Muller, T. et al. (2004) Tocopherol long chain fatty alcohols decrease the production of TNF-alpha and NO radicals by activated microglial cells.
Bioorg Med Chem Lett.14: 6023-6. -
Pazmany, T. et al. (2000) Effect of transforming growth factor-beta1 on microglial MHC-class II expression.
J Neuroimmunol. 103: 122-30. -
Himoudi, N. et al. (2007) Development of anti-PAX3 immune responses; a target for cancer immunotherapy.
Cancer Immunol Immunother. 56: 1381-95.
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