Ly-6C/CD31 | ERMP20/ERMP12

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Rat anti Mouse Ly-6C:Alexa Fluor® 488/CD31:RPE-Alexa Fluor® 647

Product Type
Dual Color Reagent
Clone
ERMP20/ERMP12
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC054 F 100 Tests
DC054 is a dual colour reagent containing antibodies which recognise murine Ly-6C and CD31 cell surface antigens. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.

Antibodies produced by clone ERMP20 recognise murine Ly-6C, a ~14 kDa differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

Antibodies produced by clone ERMP12 recognise murine CD31, a ~140 kDa cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets, and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow.

Product Details

Target Species
Mouse
Product Form
Dual Colour combination consisting of Alexa Fluor®488 conjugated and RPE-Alexa Fluor®647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
A488 reagent Rat IgG2a
RPE-A647 reagent Rat IgG2a

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P09568 Related reagents
Q08481 Related reagents
Entrez Gene
Ly6c1 Related reagents
Pecam1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0031225 anchored to membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005625 soluble fraction
GO:0007266 Rho protein signal transduction
GO:0030054 cell junction
GO:0030334 regulation of cell migration
GO:0030485 smooth muscle contractile fiber
GO:0042060 wound healing
GO:0045121 membrane raft
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Ly-6C/CD31

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Product Specific References

References for Ly-6C/CD31

  1. Nikolic, T. . (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int. Immunol.15:515-524.