Ly-6C/CD115/CD11b | ERMP20 / 604B5 2E11 / M1/70.15

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anti Mse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD11b:RPE-Alexa Fluor® 647

Product Type
Triple Color Reagent
Clone
ERMP20 / 604B5 2E11 / M1/70.15
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
TC015 F 100 Tests
Anti Mouse Ly-6C:Alexa Fluor® 488/CD115:RPE/CD11b:RPE-Alexa Fluor® 647 is a triple color reagent containing antibodies which recognize murine Ly-6C, CD115 and CD11b cell surface antigens.

Antibodies produced by clone ERMP20 recognize murine Ly-6C, a 14 kDa differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

Antibodies produced by clone 604B5 2E11 recognize the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.

Antibodies produced by clone M1/70.15 recognize the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

Product Details

Target Species
Mouse
Product Form
Triple Color combination consisting of Alexa Fluor® 488, RPE and RPE-Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
Ly-6C A488 reagent: Rat IgG2a
CD115 RPE reagent: Rat IgG1
CD11b RPE-A647 reagent: Rat IgG2b

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P09568 Related reagents
P09581 Related reagents
P05555 Related reagents
Entrez Gene
Itgam Related reagents
Ly6c1 Related reagents
Csf1r Related reagents
GO Terms
GO:0001846 opsonin binding
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0005634 nucleus
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0030593 neutrophil chemotaxis
GO:0043395 heparan sulfate proteoglycan binding
GO:0045123 cellular extravasation
GO:0050798 activated T cell proliferation
GO:0005886 plasma membrane
GO:0031225 anchored to membrane
GO:0016021 integral to membrane
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0019955 cytokine binding
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Ly-6C/CD115/CD11b

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).