Integrin Alpha 4 Beta 7 antibody | DATK32
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Rat anti Mouse integrin alpha 4 beta 7 recognizes integrin alpha 4 beta 7 also known as LPAM-1 or lymphocyte Peyer's patch adhesion molecule. Integrin alpha 4 beta 7 is composed of (~150 kDa) alpha 4 and beta 7 (~130 kDa) components. Integrin alpha 4 beta 7 and its ligand MAdCAM-1 play a vital role in the homing of activated lymphocytes to gut associated lymphoid tissues. Integrin alpha 4 beta 7 expression is found on several cell types including lymphocytes, macrophages and eosinophils.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- TK1 murine T cell lymphoma cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized fischer rats were fused with cells from the murine SP2/0-Ag14 myeloma.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q00651
- P26011
- Entrez Gene
- Itgb7
- Itga4
- GO Terms
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007275 multicellular organismal development
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001968 fibronectin binding
- GO:0001974 blood vessel remodeling
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007507 heart development
- GO:0009897 external side of plasma membrane
- GO:0016477 cell migration
- GO:0060324 face development
- GO:0060710 chorio-allantoic fusion
- Regulatory
- For research purposes only
Applications of Integrin Alpha 4 Beta 7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/20 | |
| Flow Cytometry | 1/2 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
|
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
|
Product Specific References
Source Reference
-
Andrew, D.P. et al. (1994) Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
J Immunol. 153 (9): 3847-61.
References for Integrin Alpha 4 Beta 7 antibody
-
Dolcetti, R. et al. (1997) Alpha 4 beta 7 integrin expression is associated with the leukemic evolution of human and murine T-cell lymphoblastic lymphomas.
Am J Pathol. 150 (5): 1595-605.
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