Integrin Alpha 4 Beta 7 antibody | DATK32

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Rat anti Mouse Integrin Alpha 4 Beta 7

Rat anti Mouse Integrin Alpha 4 Beta 7:RPE

Product Type
Monoclonal Antibody
Clone
DATK32
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6057GA F 0.1 mg
MCA6057PE F 100 Tests/0.5ml
Rat anti Mouse integrin alpha 4 beta 7 recognizes integrin alpha 4 beta 7 also known as LPAM-1 or lymphocyte Peyer's patch adhesion molecule. Integrin alpha 4 beta 7 is composed of (~150 kDa) alpha 4 and beta 7 (~130 kDa) components. Integrin alpha 4 beta 7 and its ligand MAdCAM-1 play a vital role in the homing of activated lymphocytes to gut associated lymphoid tissues. Integrin alpha 4 beta 7 expression is found on several cell types including lymphocytes, macrophages and eosinophils.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
TK1 murine T cell lymphoma cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized fischer rats were fused with cells from the murine SP2/0-Ag14 myeloma.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch

More Information

UniProt
Q00651 Related reagents
P26011 Related reagents
Entrez Gene
Itgb7 Related reagents
Itga4 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007275 multicellular organismal development
GO:0007157 heterophilic cell-cell adhesion
GO:0001968 fibronectin binding
GO:0001974 blood vessel remodeling
GO:0007159 leukocyte cell-cell adhesion
GO:0007507 heart development
GO:0009897 external side of plasma membrane
GO:0016477 cell migration
GO:0060324 face development
GO:0060710 chorio-allantoic fusion
Regulatory
For research purposes only

Applications of Integrin Alpha 4 Beta 7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Flow Cytometry 1/2 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Product Specific References

Source Reference

  1. Andrew, D.P. et al. (1994) Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
    J Immunol. 153 (9): 3847-61.

References for Integrin Alpha 4 Beta 7 antibody

  1. Dolcetti, R. et al. (1997) Alpha 4 beta 7 integrin expression is associated with the leukemic evolution of human and murine T-cell lymphoblastic lymphomas.
    Am J Pathol. 150 (5): 1595-605.