IL-6 antibody | MP5-32C11

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Rat anti Mouse Interleukin-6

Product Type
Monoclonal Antibody
Clone
MP5-32C11
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1491 E WB 0.5 mg
Rat anti mouse Interleukin-6 antibody, clone MP5-32C11 recognizes murine interleukin 6 (IL-6). IL-6 is a potent lymphoid cell growth factor which is principally expressed by macrophages and T-cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant mouse IL-6
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08505 Related reagents
Entrez Gene
Il6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0009897 external side of plasma membrane
GO:0031018 endocrine pancreas development
GO:0042832 defense response to protozoan
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0046716 muscle cell homeostasis
GO:0046888 negative regulation of hormone secretion
GO:0050679 positive regulation of epithelial cell proliferation
GO:0060445 branching involved in salivary gland morphogenesis
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for IL-6 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Bishop, G.A. et al. (2000) Molecular mechanisms of B lymphocyte activation by the immune response modifier R-848.
    J Immunol. 165 (10): 5552-7.
  3. Boonstra, A. et al. (2000) UVB irradiation modulates systemic immune responses by affecting cytokine production of antigen-presenting cells.
    Int Immunol. 12 (11): 1531-8.
  4. Dohi, T. et al. (2001) Elimination of colonic patches with lymphotoxin beta receptor-Ig prevents Th2 cell-type colitis.
    J Immunol. 167 (5): 2781-90.