IL-6 antibody | MP5-20F3

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Anti Mouse Interleukin-6 Antibody, clone MP5-20F3 thumbnail image 1

Anti Mouse Interleukin-6 Antibody, clone MP5-20F3 thumbnail image 2

Anti Mouse Interleukin-6 Antibody, clone MP5-20F3 gallery image 1 — **Published customer image:**
Rat anti Mouse interleukin-6 antibody, clone MP5-20F3 (**MCA1490**) used to demonstrate IL-6 expression in mouse aortic root by immunofluorescence.
**Image caption:**
Ablation of adipocyte IKKβ (inhibitor of NF‐κB [nuclear factor κB] kinase β) aggravates atherosclerotic lesional inflammation at aortic root of obese LDLR (low‐density lipoprotein receptor)–deficient (LDLR ^−/−) mice. Four‐week‐old male loxP‐flanked IKKβ LDLR ^−/− (IKKβF/FLDLR^−/−) and IKKβ‐deficient LDLR ^−/− (IKKβΔAd LDLR ^−/−) mice were fed a high‐fat diet for 12 weeks. Representative images and quantification data are shown for immunofluorescence staining at the aortic root of obese IKKβF/FLDLR^−/− and IKKβΔAd LDLR ^−/− mice (scale bars=100μm): IL‐6 (interleukin 6; n=5 each group; values are mean±SEM; **P<0.01). DAPI indicates 4',6‐diamidino‐2‐phenylindole.

From: Lu W, Park SH, Meng Z, Wang F, Zhou C.
Deficiency of Adipocyte IKKβ Affects Atherosclerotic Plaque Vulnerability in Obese LDLR Deficient Mice.
J Am Heart Assoc. 2019 Jun 18;8(12):e012009.
doi: 10.1161/JAHA.119.012009.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Mouse Interleukin-6 Antibody, clone MP5-20F3 gallery image 2 — **Published customer image:**
Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 (**MCA1490**) used to visualize IL-6 expression in murine cardiac tissue by immunofluorescence.
**Image caption:**
Representative images of immunofluorescence staining (left) and the quantifications (right) of IL-6 (C) and MCP-1 (D) in the hearts of IKKβ^fl/fl and IKKβ^ΔFib mice. The nuclei were visualized with DAPI (blue) (n = 3–4; 2-way ANOVA; ***P <0.001; scale bar: 50 μm).

From: Lu W, Meng Z, Hernandez R, Zhou C.
Fibroblast-specific IKK-β deficiency ameliorates angiotensin II-induced adverse cardiac remodeling in mice.
JCI Insight. 2021 Sep 22;6(18):e150161.
doi: 10.1172/jci.insight.150161.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Mouse Interleukin-6

Product Type: Monoclonal Antibody
Clone: MP5-20F3
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1490 Datasheet	E SDS	0.5 mg	Log in		Get Quote

Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 recognizes mouse Interleukin 6 (IL-6), also known as B-cell hybridoma growth factor or Interleukin HP-1. IL-6 is a potent lymphoid cell growth factor principally expressed by macrophages and T-cells. IL-6 is a potent inducer of the acute phase response.

Animals lacking IL-6 demonstrate a marked reduction in the production of proinflammatory Th17 cells (Ivanov et al. 2006). In humans disruption of the IL-6 gene may lead to the presentation of rheumatoid arthritis systemic juvenile (RASJ), an inflammatory articular disorder manifesting with daily spiking fever, macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 may be used to neutralize IL-6 bioactivity in a functional assay (Abrams et al. 1992 and Kambayashi et al. 1995).

Product Details

Target Species: Mouse
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant murine IL-6.
Approx. Protein Concentrations: IgG concentration 0.5mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P08505
Entrez Gene: Il6
GO Terms: GO:0001781 neutrophil apoptosis; GO:0006953 acute-phase response; GO:0005125 cytokine activity; GO:0005138 interleukin-6 receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0009897 external side of plasma membrane; GO:0031018 endocrine pancreas development; GO:0042832 defense response to protozoan; GO:0045079 negative regulation of chemokine biosynthetic process; GO:0045630 positive regulation of T-helper 2 cell differentiation; GO:0045893 positive regulation of transcription, DNA-dependent; GO:0046716 muscle cell homeostasis; GO:0046888 negative regulation of hormone secretion; GO:0050679 positive regulation of epithelial cell proliferation; GO:0060445 branching involved in salivary gland morphogenesis; GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis; GO:0070091 glucagon secretion; GO:0070102 interleukin-6-mediated signaling pathway
Regulatory: For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	C E P WB	1 ml	Log in	Get Quote
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	C E IF P WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	C E P RE	1 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	F	0.5 ml	Log in	Get Quote
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)	STAR71D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	F	0.5 ml	Log in	Get Quote

Product Specific References

References for IL-6 antibody

Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Kambayashi, T. et al. (1995) Cyclic nucleotide phosphodiesterase type IV participates in the regulation of IL-10 and in the subsequent inhibition of TNF-alpha and IL-6 release by endotoxin-stimulated macrophages.
J Immunol. 155 (10): 4909-16.
Vankelecom, H. et al. (1993) Immunocytochemical evidence that S-100-positive cells of the mouse anterior pituitary contain interleukin-6 immunoreactivity.
J Histochem Cytochem. 41 (2): 151-6.
Skok, J. et al. (1999) Dendritic cell-derived IL-12 promotes B cell induction of Th2 differentiation: a feedback regulation of Th1 development.
J Immunol. 163 (8): 4284-91.
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.
Lu, W. et al. (2019) Deficiency of Adipocyte IKKβ Affects Atherosclerotic Plaque Vulnerability in Obese LDLR Deficient Mice.
J Am Heart Assoc. 8 (12): e012009.
Lu, W. et al. (2021) Fibroblast-specific IKK-β deficiency ameliorates angiotensin II-induced adverse cardiac remodeling in mice.
JCI Insight. 6 (18)Sep 22 [Epub ahead of print].
Jia, W. et al. (2022) MircoRNA-126-5p inhibits apoptosis of endothelial cell in vascular arterial walls via NF-κB/PI3K/AKT/mTOR signaling pathway in atherosclerosis.
J Mol Histol. Jan 04 [Epub ahead of print].

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