Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3
recognizes mouse Interleukin 6 (IL-6). IL-6 is a potent lymphoid cell growth factor which is principally expressed by macrophages and T-cells.
Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 may be used to neutralize IL-6 bioactivity in a functional assay (Abrams et al. 1992
and Kambayashi et al. 1995
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant murine IL-6.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
interleukin-6 receptor binding
growth factor activity
external side of plasma membrane
endocrine pancreas development
defense response to protozoan
negative regulation of chemokine biosynthetic process
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-dependent
muscle cell homeostasis
negative regulation of hormone secretion
positive regulation of epithelial cell proliferation
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
interleukin-6-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-6 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IL-6 antibody
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Kambayashi, T. et al. (1995) Cyclic nucleotide phosphodiesterase type IV participates in the regulation of IL-10 and in the subsequent inhibition of TNF-alpha and IL-6 release by endotoxin-stimulated macrophages.
J Immunol. 155 (10): 4909-16.
Vankelecom, H. et al. (1993) Immunocytochemical evidence that S-100-positive cells of the mouse anterior pituitary contain interleukin-6 immunoreactivity.
J Histochem Cytochem. 41 (2): 151-6.
Skok, J. et al. (1999) Dendritic cell-derived IL-12 promotes B cell induction of Th2 differentiation: a feedback regulation of Th1 development.
J Immunol. 163 (8): 4284-91.
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.