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IL-6 antibody | MP5-20F3

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Anti Mouse Interleukin-6 Antibody, clone MP5-20F3 gallery image 1
Published customer image:
Rat anti Mouse interleukin-6 antibody, clone MP5-20F3 (MCA1490) used to demonstrate IL-6 expression in mouse aortic root by immunofluorescence.
Image caption:
Ablation of adipocyte IKKβ (inhibitor of NF‐κB [nuclear factor κB] kinase β) aggravates atherosclerotic lesional inflammation at aortic root of obese LDLR (low‐density lipoprotein receptor)–deficient (LDLR −/−) mice. Four‐week‐old male loxP‐flanked IKKβ LDLR −/− (IKKβF/FLDLR−/−) and IKKβ‐deficient LDLR −/− (IKKβΔAd LDLR −/−) mice were fed a high‐fat diet for 12 weeks. Representative images and quantification data are shown for immunofluorescence staining at the aortic root of obese IKKβF/FLDLR−/− and IKKβΔAd LDLR −/− mice (scale bars=100μm): IL‐6 (interleukin 6; n=5 each group; values are mean±SEM; **P<0.01). DAPI indicates 4',6‐diamidino‐2‐phenylindole.

From: Lu W, Park SH, Meng Z, Wang F, Zhou C.
Deficiency of Adipocyte IKKβ Affects Atherosclerotic Plaque Vulnerability in Obese LDLR Deficient Mice.
J Am Heart Assoc. 2019 Jun 18;8(12):e012009.
doi: 10.1161/JAHA.119.012009.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti Mouse Interleukin-6

Product Type
Monoclonal Antibody
Clone
MP5-20F3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1490
Datasheet
E
MSDS
0.5 mg loader

Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 recognizes mouse Interleukin 6 (IL-6), also known as B-cell hybridoma growth factor or Interleukin HP-1. IL-6 is a potent lymphoid cell growth factor principally expressed by macrophages and T-cells. IL-6 is a potent inducer of the acute phase response.

Animals lacking IL-6 demonstrate a marked reduction in the production of proinflammatory Th17 cells (Ivanov et al. 2006). In humans disruption of the IL-6 gene may lead to the presentation of rheumatoid arthritis systemic juvenile (RASJ), an inflammatory articular disorder manifesting with daily spiking fever, macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Rat anti Mouse Interleukin-6 antibody, clone MP5-20F3 may be used to neutralize IL-6 bioactivity in a functional assay (Abrams et al. 1992 and Kambayashi et al. 1995).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant murine IL-6.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08505
Entrez Gene
Il6
GO Terms
GO:0001781 neutrophil apoptosis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0009897 external side of plasma membrane
GO:0031018 endocrine pancreas development
GO:0042832 defense response to protozoan
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0046716 muscle cell homeostasis
GO:0046888 negative regulation of hormone secretion
GO:0050679 positive regulation of epithelial cell proliferation
GO:0060445 branching involved in salivary gland morphogenesis
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Product Specific References

References for IL-6 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Kambayashi, T. et al. (1995) Cyclic nucleotide phosphodiesterase type IV participates in the regulation of IL-10 and in the subsequent inhibition of TNF-alpha and IL-6 release by endotoxin-stimulated macrophages.
    J Immunol. 155 (10): 4909-16.
  3. Vankelecom, H. et al. (1993) Immunocytochemical evidence that S-100-positive cells of the mouse anterior pituitary contain interleukin-6 immunoreactivity.
    J Histochem Cytochem. 41 (2): 151-6.
  4. Skok, J. et al. (1999) Dendritic cell-derived IL-12 promotes B cell induction of Th2 differentiation: a feedback regulation of Th1 development.
    J Immunol. 163 (8): 4284-91.
  5. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Curr Alzheimer Res. 6: 531-40.
  6. Lu, W. et al. (2019) Deficiency of Adipocyte IKKβ Affects Atherosclerotic Plaque Vulnerability in Obese LDLR Deficient Mice.
    J Am Heart Assoc. 8 (12): e012009.
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