IL-6 antibody | 6B4 IGH 54

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Rat anti Mouse Interleukin-6:Preservative Free

Product Type
Monoclonal Antibody
Clone
6B4 IGH 54
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2794Z
Datasheet
E FN
MSDS
0.1 mg loader

Rat anti Mouse Interleukin-6 antibody, clone 6B4 IGH 54recognizes mouse IL-6 (interleukin-6), a multifunctional pro-inflammatory cytokine secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts acts as the primary inducer of the acute phase response in the liver.

IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Ra chain (CD126) and the signal-transducing component gp130 (CD130). IL-6 can also stimulate cells expressing gp130 through a soluble IL-6R. IL-6 has been shown to stimulate B cell differentiation and subsequent immunoglobulin production, promote hybridoma growth, as well as regulating bone metabolism and stimulating energy mobilisation in muscle and fatty tissue.

Rat anti Mouse Interleukin-6 antibody, clone 6B4 IGH 54 can be used to neutralize the activity of 50pg/ml of recombinant mouse IL-6 by >95% in a functional assay.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Natural partially purified mouse IL-6.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Endotoxin Level
< 0.004 EU/ug

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08505 Related reagents
Entrez Gene
Il6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0009897 external side of plasma membrane
GO:0031018 endocrine pancreas development
GO:0042832 defense response to protozoan
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0046716 muscle cell homeostasis
GO:0046888 negative regulation of hormone secretion
GO:0050679 positive regulation of epithelial cell proliferation
GO:0060445 branching involved in salivary gland morphogenesis
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Product Specific References

Further Reading

  1. Peters, M. et al. (1998) Interleukin-6 and soluble interleukin-6 receptor: direct stimulation of gp130 and hematopoiesis.
    Blood. 92 (10): 3495-504.