IL-6 antibody | 6B4 IGH 54
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Rat anti Mouse Interleukin-6 antibody, clone 6B4 IGH 54recognizes mouse IL-6 (interleukin-6), a multifunctional pro-inflammatory cytokine secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts acts as the primary inducer of the acute phase response in the liver.
IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Ra chain (CD126) and the signal-transducing component gp130 (CD130). IL-6 can also stimulate cells expressing gp130 through a soluble IL-6R. IL-6 has been shown to stimulate B cell differentiation and subsequent immunoglobulin production, promote hybridoma growth, as well as regulating bone metabolism and stimulating energy mobilisation in muscle and fatty tissue.
Rat anti Mouse Interleukin-6 antibody, clone 6B4 IGH 54 can be used to neutralize the activity of 50pg/ml of recombinant mouse IL-6 by >95% in a functional assay.
IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Ra chain (CD126) and the signal-transducing component gp130 (CD130). IL-6 can also stimulate cells expressing gp130 through a soluble IL-6R. IL-6 has been shown to stimulate B cell differentiation and subsequent immunoglobulin production, promote hybridoma growth, as well as regulating bone metabolism and stimulating energy mobilisation in muscle and fatty tissue.
Rat anti Mouse Interleukin-6 antibody, clone 6B4 IGH 54 can be used to neutralize the activity of 50pg/ml of recombinant mouse IL-6 by >95% in a functional assay.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Natural partially purified mouse IL-6.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Endotoxin Level
- < 0.004 EU/ug
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P08505
- Entrez Gene
- Il6
- GO Terms
- GO:0001781 neutrophil apoptosis
- GO:0006953 acute-phase response
- GO:0005125 cytokine activity
- GO:0005138 interleukin-6 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0009897 external side of plasma membrane
- GO:0031018 endocrine pancreas development
- GO:0042832 defense response to protozoan
- GO:0045079 negative regulation of chemokine biosynthetic process
- GO:0045630 positive regulation of T-helper 2 cell differentiation
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0046716 muscle cell homeostasis
- GO:0046888 negative regulation of hormone secretion
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0060445 branching involved in salivary gland morphogenesis
- GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
- GO:0070091 glucagon secretion
- GO:0070102 interleukin-6-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of IL-6 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays | 3ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Product Specific References
Further Reading
-
Peters, M. et al. (1998) Interleukin-6 and soluble interleukin-6 receptor: direct stimulation of gp130 and hematopoiesis.
Blood. 92 (10): 3495-504.
