IL-4 antibody | BVD6-24G2

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Rat anti Mouse Interleukin-4

Product Type: Monoclonal Antibody
Clone: BVD6-24G2
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1492 Datasheet	C E F* SDS	0.5 mg	Log in		Get Quote

Rat anti Mouse Interleukin-4 antibody, clone BVD6-24G2 recognizes mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid cell growth factor, which stimulates the growth and survival of certain B and T cells.

Rat anti Mouse Interleukin-4 antibody, clone BVD6-24G2 is not reported to have neutralising activity.

Product Details

Target Species

Mouse

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Recombinant murine IL-4.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P07750
Entrez Gene: Il4
GO Terms: GO:0005125 cytokine activity; GO:0005136 interleukin-4 receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0008203 cholesterol metabolic process; GO:0030890 positive regulation of B cell proliferation; GO:0042104 positive regulation of activated T cell proliferation; GO:0042113 B cell activation; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0043066 negative regulation of apoptosis; GO:0043193 positive regulation of gene-specific transcription; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048295 positive regulation of isotype switching to IgE isotypes; GO:0048304 positive regulation of isotype switching to IgG isotypes; GO:0051091 positive regulation of transcription factor activity
Regulatory: For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5ug/ml	2ug/ml
Flow Cytometry ¹
Immunohistology - Frozen

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	C E P WB	1 ml	Log in	Get Quote
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	C E IF P WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	C E P RE	1 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	F	0.5 ml	Log in	Get Quote
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	F	0.5 ml	Log in	Get Quote

Product Specific References

References for IL-4 antibody

Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Bogen, S.A. et al. (1993) Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
J Immunol. 150 (10): 4197-205.
Fujihashi, K. et al. (1993) Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells.
J Immunol Methods. 160 (2): 181-9.
Ferrick, D.A. et al. (1995) Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo.
Nature. 373 (6511): 255-7.
Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 2009 Apr;20(4):254-60.

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