Rat anti Mouse Interleukin-4 antibody, clone BVD6-24G2 recognizes mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid cell growth factor, which stimulates the growth and survival of certain B and T cells.
Rat anti Mouse Interleukin-4 antibody, clone BVD6-24G2 is not reported to have neutralising activity.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Recombinant murine IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
interleukin-4 receptor binding
growth factor activity
cholesterol metabolic process
positive regulation of B cell proliferation
positive regulation of activated T cell proliferation
B cell activation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptosis
positive regulation of gene-specific transcription
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
positive regulation of transcription factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-4 antibody
|Flow Cytometry 1
|Immunohistology - Frozen
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for IL-4 antibody
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Bogen, S.A. et al. (1993) Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
J Immunol. 150 (10): 4197-205.
Fujihashi, K. et al. (1993) Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells.
J Immunol Methods. 160 (2): 181-9.
Ferrick, D.A. et al. (1995) Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo.
Nature. 373 (6511): 255-7.
Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 2009 Apr;20(4):254-60.
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