IgG3 antibody | AbD04582
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Human anti Mouse IgG3:FITC
- Product Type
- Monoclonal Antibody
- Clone
- AbD04582
- Isotype
- HuCAL Fab bivalent
- Specificity
- IgG3
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Antibody Quality and 
Unrivaled 
| Human anti Mouse IgG3 antibody, clone AbD04582 recognizes mouse IgG3. No cross reactivity is seen with other mouse immunoglobulin sub-classes, rat immunoglobulins, or sheep, bovine and goat IgG. |
- Target Species
- Mouse
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and is conjugated to fluorescein isothiocyanate (FITC).
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- Purified mouse monoclonal IgG3.
- Approx. Protein Concentrations
- Antibody concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
- RRID
- AB_999303
- UniProt
- P03987
- Entrez Gene
- AI324046
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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