IgG3 antibody | AbD04582

Human anti Mouse IgG3:FITC

Product Type
Monoclonal Antibody
Clone
AbD04582
Isotype
HuCAL Fab bivalent
Specificity
IgG3

Product Code Applications Pack Size List Price Your Price Qty
HCA039F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
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Human anti Mouse IgG3 antibody, clone AbD04582 recognizes mouse IgG3. No cross reactivity is seen with other mouse immunoglobulin sub-classes, rat immunoglobulins, or sheep, bovine and goat IgG.

Target Species
Mouse
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and is conjugated to fluorescein isothiocyanate (FITC).
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Purified mouse monoclonal IgG3.
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

RRID
AB_999303
UniProt
P03987
Entrez Gene
AI324046
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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