IgG2b antibody | LO-MG2b-2
Biotin labeled Rat anti Mouse IgG2b Heavy Chain antibody, clone LO-MG2b-2 (MCA422B) used for antibody class evaluation by ELISA.
Image caption:
Anti-IGF1R and anti-HER2/neu antibodies elicited by cell vaccines co-targeting HER2/neu and IGF1R.
Analysis of antibody isotypes induced by cell vaccines as in Figure 3A (n = 3 mice per group).
From: De Giovanni C, Landuzzi L, Palladini A, Ianzano ML, Nicoletti G, Ruzzi F, Amici A, Croci S, Nanni P, Lollini PL.
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 2019 Apr 11;11(4). pii: E517.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
E ResetRat anti Mouse IgG2b Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG2b-2
- Isotype
- IgG1
- Specificity
- IgG2b
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgG2b Heavy Chain antibody, clone LO-MG2b-2 reacts with mouse IgG2b heavy chain. The avidity of Rat anti Mouse IgG2b Heavy Chain antibody, clone LO-MG2b-2 for IgG2b is 1 x 1010 M-1. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified mouse IgG2b.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.25ug/ml (detection) |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG2b antibody
-
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J. Immunol. Methods 71: 261-269. -
Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
Int. Immunol. 5: 71-8. -
DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 11 (4)Apr 11 [Epub ahead of print].
- RRID
- AB_321834
- UniProt
- P01867
- Entrez Gene
- Ighg2b
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
MCA422
060815
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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