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IgG2b antibody | LO-MG2b-2

Rat anti Mouse IgG2b Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG2b-2
Isotype
IgG1
Specificity
IgG2b
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA422
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 mg loader
List Price Your Price
loader

Rat anti Mouse IgG2b Heavy Chain antibody, clone LO-MG2b-2 reacts with mouse IgG2b heavy chain. The avidity of Rat anti Mouse IgG2b Heavy Chain antibody, clone LO-MG2b-2 for IgG2b is 1 x 1010 M-1.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified mouse IgG2b.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.25ug/ml (detection)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for IgG2b antibody

  1. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J Immunol Methods. 66 (2): 261-9.
  2. Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
    Int. Immunol. 5: 71-8.
  3. DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
    Cancers (Basel). 11 (4)Apr 11 [Epub ahead of print].

RRID
AB_321834
UniProt
P01867
Entrez Gene
Ighg2b
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region

MCA422

060815

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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