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IgG2a antibody | LO-MG2a-9

Rat anti Mouse IgG2a Heavy Chain:HRP

Product Type
Monoclonal Antibody
Clone
LO-MG2a-9
Isotype
IgG1
Specificity
IgG2a
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA1588P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader

Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 recognizes the mouse gamma 2a heavy chain, binding to all allotypes and does not cross-react with other murine immunoglobulin classes or subclasses.

The avidity of Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 for murine IgG2a = 2.5 x 109M-1.

Target Species
Mouse
Product Form
Purified IgG conjugated to horseradish peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
50% glycerol
Immunogen
Purified IgG2a from Balb/c mice. PTR
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 250ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG2a antibody

  1. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
    Vaccine. 24: 4102-10.
  2. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels.
    J Allergy Clin Immunol. 122: 633-9.e3.
  3. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  4. Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
    PLoS One. 9 (6): e100392.
  5. Carew, J.S. et al. (2015) Targeting Survivin Inhibits Renal Cell Carcinoma Progression and Enhances the Activity of Temsirolimus.
    Mol Cancer Ther. 14 (6): 1404-13.
  6. Islam, S. et al. (2020) Resistance to histone deacetylase inhibitors confers hypersensitivity to oncolytic reovirus therapy.
    Blood Adv. 4 (20): 5297-310.

ELISA

RRID
AB_322872
UniProt
P01865
P01864
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane

MCA1588P

153791 160262 163881 180117

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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