IgE antibody | LO-ME-3

Rat anti Mouse IgE Heavy Chain:FITC

Product Type
Monoclonal Antibody
Clone
LO-ME-3
Isotype
IgG1
Specificity
IgE
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA419F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 is = 3 x 109M-1

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Immunogen
Purified IgE from BALB/c mice.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

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References for IgE antibody

  1. Mojtabavi, N. et al. (2002) Long-lived Th2 memory in experimental allergic asthma.
    J Immunol. 169 (9): 4788-96.
  2. Stevens,T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust
    Toxicol.Sci. 102: 359-70.
  3. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  4. Bemark, M. et al. (2011) A unique role of the cholera toxin A1-DD adjuvant for long-term plasma and memory B cell development.
    J Immunol. 186: 1399-410.
  5. Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
    J Immunol. 174 (1): 525-32.
  6. Komai, M. et al. (2010) A novel CC-chemokine receptor 3 antagonist, Ki19003, inhibits airway eosinophilia and subepithelial/peribronchial fibrosis induced by repeated antigen challenge in mice.
    J Pharmacol Sci. 112: 203-13.
  7. Komai, M. et al. (2003) Role of Th2 responses in the development of allergen-induced airway remodelling in a murine model of allergic asthma.
    Br J Pharmacol. 138: 912-20
  8. Lee, M.Y. et al. (2011) Protective Effects of Mentha haplocalyx Ethanol Extract (MH) in a Mouse Model of Allergic Asthma.
    Phytother Res. 25(6):863-9.
  9. View The Latest Product References
  10. Niwa, S. et al. (2010) Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
    Lipids Health Dis. 9:1.
  11. Hazebrouck, S. et al. (2009) Allergic sensitization to bovine beta-lactoglobulin: comparison between germ-free and conventional BALB/c mice.
    Int Arch Allergy Immunol. 148: 65-72.
  12. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  13. Kalenda, Y.D. et al. (2015) Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection.
    Parasitol Int. 64 (6): 503-12.
  14. Kim, J.H. & Ohsawa, M. (1995) Oral tolerance to ovalbumin in mice as a model for detecting modulators of the immunologic tolerance to a specific antigen.
    Biol Pharm Bull. 18 (6): 854-8.
  15. Ray, A. et al. (2015) Gut Microbial Dysbiosis Due to Helicobacter Drives an Increase in Marginal Zone B Cells in the Absence of IL-10 Signaling in Macrophages.
    J Immunol. 195 (7): 3071-85.
  16. Nakano, Y. et al. (2016) Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice.
    J Pharmacol Exp Ther. 357 (2): 258-63.
  17. Jeon, W.Y. et al. (2015) Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
    BMC Complement Altern Med. 15 (1): 329.
  18. Ziegler, T. et al. (2015) A novel regulatory macrophage induced by a helminth molecule instructs IL-10 in CD4+ T cells and protects against mucosal inflammation.
    J Immunol. 194 (4): 1555-64.
  19. Noble A & Zhao J (2016) Follicular helper T cells are responsible for IgE responses to Der p 1 following house dust mite sensitization in mice.
    Clin Exp Allergy. May 3 [Epub ahead of print]
  20. Roberts, H. et al. (2016) Characterization of neutrophil function in Papillon-Lefèvre syndrome.
    J Leukoc Biol. 100 (2): 433-44.
  21. Ikeda, M. et al. (2016) Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model of chronic asthma.
    Allergol Int. pii: S1323-8930(16)30161-7. [Epub ahead of print]
  22. González-Chomón C et al. (2016) Biomimetic contact lenses eluting olopatadine for allergic conjunctivitis.
    Acta Biomater. 41: 302-11.
  23. Maiga, M.A. et al. (2017) Neonatal mono-colonization of germ-free mice with Lactobacillus casei enhances casein immunogenicity after oral sensitization to cow's milk.
    Mol Nutr Food Res. Mar 20. [Epub ahead of print]
  24. J Wadley, A. et al. (2017) Heightened Exercise-Induced Oxidative Stress at Simulated Moderate Level Altitude vs. Sea Level in Trained Cyclists.
    Int J Sport Nutr Exerc Metab. 27 (2): 97-104.
  25. Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
    J Exp Med. May 8. pii: jem.20161807.
  26. Chesné, J. et al. (2015) Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite-induced allergic asthma model.
    J Allergy Clin Immunol. 135 (6): 1643-1643.e3.
  27. Di, C. et al. (2015) Basophil-associated OX40 ligand participates in the initiation of Th2 responses during airway inflammation.
    J Biol Chem. 290 (20): 12523-36.
  28. Mohammed, E.S. et al.. (2020) Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.
    PLoS Negl Trop Dis. 2020;14(9):e0008518.
  29. Yamashita, H. et al. (2017) Artificial sweeteners and mixture of food additives cause to break oral tolerance and induce food allergy in murine oral tolerance model for food allergy.
    Clin Exp Allergy. 47 (9): 1204-13.
  30. Yamashita, H. et al. (2021) Impact of orally-administered oligosaccharides in a murine model of food allergy
    Journal of Functional Foods. 85: 104643.
  31. Ghonim, M.A. et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
    J Transl Med. 16 (1): 243.

ELISA

RRID
AB_321897
UniProt
P06336
Entrez Gene
Gm900
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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