IgE antibody | AbD04658

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Anti Mouse IgE Antibody, clone AbD04658 thumbnail image 1

Anti Mouse IgE Antibody, clone AbD04658 gallery image 1 — Mouse IgE detected using Human anti Mouse IgE (**HCA058A**).

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Human anti Mouse IgE

Product Type: Monoclonal Antibody
Clone: AbD04658
Isotype: HuCAL Fab bivalent
Specificity: IgE

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Product Code Applications Pack Size List Price Your Price Qty

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HCA058A Datasheet Datasheet SDS SDS	E	50 µg		Log in	Get Quote
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Human anti Mouse IgE antibody, clone AbD04658 recognizes mouse IgE. No cross reactivity is seen with other mouse immunoglobulin sub-classes or rat, sheep, bovine and goat immunoglobulins.

Target Species: Mouse
Product Form: A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Purified mouse monoclonal IgE.
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 6 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/1000	1/5000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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RRID: AB_915308
UniProt: P06336
Entrez Gene: Gm900
GO Terms: GO:0003823 antigen binding
Licensed Use: For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.