Gr-1 antibody | RB6-8C5




































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F IF P ResetRat anti Mouse Gr-1:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- RB6-8C5
- Isotype
- IgG2b
- Specificity
- Gr-1
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a ~21–25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Rat anti Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007) The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Rat anti Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (Czuprynski et al 1994, Daley et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Normal murine bone marrow cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR |
References for Gr-1 antibody
-
Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.
J Immunol. 147 (1): 22-8. -
Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
J Immunol. 151 (5): 2399-408. -
Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.
J Immunol. 152 (4): 1836-46. -
Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.
J Exp Med. 179:259-68. -
Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.
Eur J Immunol. 32 (11): 3235-45. -
Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Guérin immunotherapy of bladder cancer and orchestrate local immune responses.
Cancer Res. 66: 8250-7. -
Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
PLoS Pathog. 3: e193. -
Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.
J Immunol. 185 (11): 7057-66.
View The Latest Product References
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Blood.117: 1734-44. -
Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
J Histochem Cytochem. 59: 813-25. -
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Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.
Endocrinology. 152: 3155-64. -
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Infect Immun. 82 (1): 253-64. -
Leblond, A.L. et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS One. 10 (9): e0137515. -
Wang, Y. et al. (2015) Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
Kidney Int. 88 (6): 1274-1282. -
Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
J Clin Invest. 125 (11): 4281-94. -
Lee, Y.S. et al. (2015) Interleukin-1 (IL-1) signaling in intestinal stromal cells controls KC/ CXCL1 secretion, which correlates with recruitment of IL-22- secreting neutrophils at early stages of Citrobacter rodentium infection.
Infect Immun. 83 (8): 3257-67. -
Roche, J.A. et al. (2015) Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient a/j mouse skeletal muscle.
Am J Pathol. 185 (6): 1686-98. -
Kojo, K. et al. (2016) BLT1 signalling protects the liver against acetaminophen hepatotoxicity by preventing excessive accumulation of hepatic neutrophils.
Sci Rep. 6: 29650. -
Cousins, F.L. et al. (2016) Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
Sci Rep. 6: 36748. -
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Neuroscience. 343: 483-94. -
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PLoS One. 13 (10): e0205437. -
Qin, X. et al. (2018) Caspase recruitment domain-containing protein 9 (CARD9) knockout reduces regional ischemia/reperfusion injury through an attenuated inflammatory response.
PLoS One. 13 (6): e0199711. -
Zhang, M.Z. et al. (2019) The Role of the EGF Receptor in Sex Differences in Kidney Injury.
J Am Soc Nephrol. 30 (9): 1659-73. -
Konishi, T. et al. (2019) Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
Am J Physiol Gastrointest Liver Physiol. 317 (6): G773-G783. -
Kamata, M. et al. (2019) Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.
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Elife. 9: e59520. -
Takahashi, R. et al. (2020) Microsomal prostaglandin E synthase-1 promotes lung metastasis via SDF-1/CXCR4-mediated recruitment of CD11b+Gr1+MDSCs from bone marrow.
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Infect Immun. 91 (5): e0001523. -
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bioRχiv 2024.07.11.602963 [Epub ahead of print].
- Synonyms
- Ly-6G
- RRID
- AB_567107
- UniProt
- P35461
- Entrez Gene
- Ly6g
- GO Terms
- GO:0009897 external side of plasma membrane
- GO:0031225 anchored to membrane
MCA2387A488



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