GITRL antibody | YGL386

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Rat anti Mouse GITRL

Product Type
Monoclonal Antibody
Clone
YGL386
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2417GA E F 0.1 mg
Rat anti Mouse GITRL antibody, clone YGL386 recognizes mouse glucocorticoid-induced tumour necrosis factor receptor ligand (GITRL), a 20 kDa member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18.

Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation.

Studies demonstrate that GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naïve and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%sodium azide
Carrier Free
Yes
Immunogen
Recombinant mouse GITRL.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UNG2 Related reagents
Entrez Gene
TNFSF18 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006955 immune response
GO:0007267 cell-cell signaling
Regulatory
For research purposes only

Applications of GITRL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA1211 1 ml C E F

Product Specific References

References for GITRL antibody

  1. Cobbold, S.P. et al. (2004) Induction of foxP3+ regulatory T cells in the periphery of T cell receptor transgenic mice tolerized to transplants.
    J Immunol. 172 (10): 6003-10.
  2. Sakihama, T. et al. (2008) A simple detection method for low-affinity membrane protein interactions by baculoviral display.
    PLoS One. 3 (12): e4024.
  3. Tone, M. et al. (2003) Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.
    Proc Natl Acad Sci U S A. 100 (25): 15059-64.