Forssman Glycosphingolipid antibody | FOM-1

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Rat anti Mouse Forssman Glycosphingolipid

Product Type
Monoclonal Antibody
Clone
FOM-1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0500-0098 C P 0.5 ml
Rat anti Forssmann glycosphingolipid antibody, clone FOM-1 recognizes Forssman glycosphingolipid (FO GSL).

FO GSL is a glycosphingolipid differentiation antigen, present on macrophages in the red pulp of the spleen, and the medulla of inguinal and axial lymph nodes. FO GSL is also present on reticular cells in the T-cell area of the spleen and lymph nodes. A subpopulation of FOM-1 positive macrophages also appear in the peritoneal cavity in response to inflammatory stimuli. Monocytes, granulocytes and other macrophages in lymphoid and non-lymphoid organs are not stained by Rat anti Forssmann glycosphingolipid antibody, clone FOM-1.
Rat anti Forssmann glycosphingolipid antibody, clone FOM-1 also recognises a marker present on sheep erythrocytes.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid.
Preparation
Purified IgM prepared by dialysis and gel filtration
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Forsssman glycosphingolipid.
Approx. Protein Concentrations
0.2 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Forssman Glycosphingolipid antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/400
Immunohistology - Paraffin 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse spleen

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Forssman Glycosphingolipid antibody

  1. Izumi, K, and Sawada, M.T. (2001) A novel assay method for glycosphingolipid deacylase by enzyme-linked immunochemical detection of lysoglycosphingolipid.
    Lipids. 36: 97-101.