ER-MP54 antibody | ER-MP54

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Rat anti Mouse ER-MP54:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
ER-MP54
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2390A488 F* 100 Tests/1ml
Rat anti Mouse ER-MP54 antibody, clone ER-MP54 recognizes a murine antigen designated ER-MP54. The ER-MP54 antigen is primarily expressed within myeloid precursor cells in the bone marrow. The antigen may be detected in immature macrophage precursors up to the monoblast stage of myeloid differentiation. Some cell lines have also been reported to express the ER-MP54 antigen at the cell surface.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor®488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Balb/c macrophage precursor cell hybrids.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of ER-MP54 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/5
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucopermâ„¢ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:Alexa Fluor® 488 MCA1211A488 100 Tests/1ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for ER-MP54 antibody

  1. Leenen, P.J.M. et.al. (1990) Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20:27 – 34.
  2. Leenen, P.J. et al. (1994) Markers of mouse macrophage development detected by monoclonal antibodies.
    J Immunol Methods. 174 (1-2): 5-19.
  3. Hung, S.I. et al. (2003) Transient expression of Ym1, a heparin-binding lectin, during developmental hematopoiesis and inflammation.
    J Leukoc Biol. 72: 72-82.
  4. Wang, H. et al. (2009) Key role of macrophages in the pathogenesis of CD18 hypomorphic murine model of psoriasis.
    J Invest Dermatol. 129 (5): 1100-14.