ER-HR3 antibody | ER-HR3
|Rat anti Mouse ER-HR3, clone ER-HR3 recognizes the murine antigen ER-HR3, a cell surface antigen expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells located predominantly in haematopoietic and lymphoid organs. ER-HR3 demonstrates very low levels of expression on peripheral blood monocytes.
During foetal development, ER-HR3 positive cells are localized to haemapoietic islands and are often associated with erythroid progenitor cells. ER-HR3 antigen may be involved in adult erythropoiesis and in the regulation of the immune response (de Jong et al. 1994).
Rat anti Mouse ER-HR3, clone ER-HR3 does not inhibit antigen-specific T-cell proliferation.
Rat anti Mouse ER-HR3, clone ER-HR3 recognizes two bands of ~69 kDa and a minor one of ~55 kDa under non-reducing conditions (de Jong et al. 1994).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Adherent F1 (CBAxBL) bone marrow stormal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3 - X63 - Ag8.563 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Antibody Characterization Reference
DeJong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. II. Biochemical and functional aspects of the ER-HR3 antigen.
Cell Tissue Res. 275 (3): 577-85. Cell Tissue Res. 275 (3): 577-85.
References for ER-HR3 antibody
de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.
Cell Tissue Res. 275: 567-76.
Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
J Leukoc Biol. 74: 857-67.
Throsby, M. et al. (2000) CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.
J Immunol. 165:1965-75.
Grabbe, S. et al. (2002) Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.
J Clin Invest. 109: 183-92.
Sonoda, Y. and Sasaki, K. (2012) Hepatic extramedullary hematopoiesis and macrophages in the adult mouse: histometrical and immunohistochemical studies.
Cells Tissues Organs. 196: 555-64.
Jacobsen, R.N. et al. (2014) Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80+VCAM1+CD169+ER-HR3+Ly6G+ erythroid island macrophages in the mouse.
Exp Hematol. pii: S0301-472X(14)00139-8.
Vogel, J. et al. (2003) Transgenic mice overexpressing erythropoietin adapt to excessive erythrocytosis by regulating blood viscosity.
Blood. 102 (6): 2278-84.
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