ER-HR3 antibody | ER-HR3
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Rat anti Mouse ER-HR3
- Product Type
- Monoclonal Antibody
- Clone
- ER-HR3
- Isotype
- IgG2c
- Specificity
- ER-HR3
Antibody Quality and Performance Guarantee
| Rat anti Mouse ER-HR3, clone ER-HR3 recognizes the murine antigen ER-HR3, a cell surface antigen expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells located predominantly in haematopoietic and lymphoid organs. ER-HR3 demonstrates very low levels of expression on peripheral blood monocytes. During foetal development, ER-HR3 positive cells are localized to haemapoietic islands and are often associated with erythroid progenitor cells. ER-HR3 antigen may be involved in adult erythropoiesis and in the regulation of the immune response (de Jong et al. 1994). Rat anti Mouse ER-HR3, clone ER-HR3 does not inhibit antigen-specific T-cell proliferation. Rat anti Mouse ER-HR3, clone ER-HR3 recognizes two bands of ~69 kDa and a minor one of ~55 kDa under non-reducing conditions (de Jong et al. 1994). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Adherent F1 (CBAxBL) bone marrow stormal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3 - X63 - Ag8.563 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Antibody Characterization Reference
-
DeJong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. II. Biochemical and functional aspects of the ER-HR3 antigen.
Cell Tissue Res. 275 (3): 577-85. Cell Tissue Res. 275 (3): 577-85.
References for ER-HR3 antibody
-
de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.
Cell Tissue Res. 275: 567-76. -
Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
J Leukoc Biol. 74: 857-67. -
Throsby, M. et al. (2000) CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.
J Immunol. 165:1965-75. -
Grabbe, S. et al. (2002) Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.
J Clin Invest. 109: 183-92. -
Sonoda, Y. and Sasaki, K. (2012) Hepatic extramedullary hematopoiesis and macrophages in the adult mouse: histometrical and immunohistochemical studies.
Cells Tissues Organs. 196: 555-64. -
Jacobsen, R.N. et al. (2014) Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80+VCAM1+CD169+ER-HR3+Ly6G+ erythroid island macrophages in the mouse.
Exp Hematol. pii: S0301-472X(14)00139-8. -
Vogel, J. et al. (2003) Transgenic mice overexpressing erythropoietin adapt to excessive erythrocytosis by regulating blood viscosity.
Blood. 102 (6): 2278-84.
- RRID
- AB_872053
MCA2393T
162159
1708
MCA2393
161042
1709
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