ER-HR3 antibody | ER-HR3

Rat anti Mouse ER-HR3

Product Type
Monoclonal Antibody
Clone
ER-HR3
Isotype
IgG2c
Specificity
ER-HR3

Product Code Applications Pack Size List Price Your Price Qty
MCA2393T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 25 µg loader
List Price Your Price
loader
MCA2393
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse ER-HR3, clone ER-HR3 recognizes the murine antigen ER-HR3, a cell surface antigen expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells located predominantly in haematopoietic and lymphoid organs. ER-HR3 demonstrates very low levels of expression on peripheral blood monocytes.

During foetal development, ER-HR3 positive cells are localized to haemapoietic islands and are often associated with erythroid progenitor cells. ER-HR3 antigen may be involved in adult erythropoiesis and in the regulation of the immune response (de Jong et al. 1994).

Rat anti Mouse ER-HR3, clone ER-HR3 does not inhibit antigen-specific T-cell proliferation.

Rat anti Mouse ER-HR3, clone ER-HR3 recognizes two bands of ~69 kDa and a minor one of ~55 kDa under non-reducing conditions (de Jong et al. 1994).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Adherent F1 (CBAxBL) bone marrow stormal cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the mouse P3 - X63 - Ag8.563 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Antibody Characterization Reference

  1. DeJong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. II. Biochemical and functional aspects of the ER-HR3 antigen.
    Cell Tissue Res. 275 (3): 577-85. Cell Tissue Res. 275 (3): 577-85.

References for ER-HR3 antibody

  1. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.
    Cell Tissue Res. 275: 567-76.
  2. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
    J Leukoc Biol. 74: 857-67.
  3. Throsby, M. et al. (2000) CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.
    J Immunol. 165:1965-75.
  4. Grabbe, S. et al. (2002) Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.
    J Clin Invest. 109: 183-92.
  5. Sonoda, Y. and Sasaki, K. (2012) Hepatic extramedullary hematopoiesis and macrophages in the adult mouse: histometrical and immunohistochemical studies.
    Cells Tissues Organs. 196: 555-64.
  6. Jacobsen, R.N. et al. (2014) Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80+VCAM1+CD169+ER-HR3+Ly6G+ erythroid island macrophages in the mouse.
    Exp Hematol. pii: S0301-472X(14)00139-8.
  7. Vogel, J. et al. (2003) Transgenic mice overexpressing erythropoietin adapt to excessive erythrocytosis by regulating blood viscosity.
    Blood. 102 (6): 2278-84.

Immunohistology - Frozen

RRID
AB_872053
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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