Dendritic/Interdigitating Cells antibody | MIDC-8
Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 does not stain thymic epithelial cells which are the major contaminants in thymic dendritic cell preparations. The antigen is also absent in blood cells, bone marrow cells and macrophages isolated from the peritoneal cavity.
The antigen recognized by Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 is found in interdigitating cells in T cell dependent areas of secondary lymphoid organs, the medulla of thymus, veiled cells, and very weakly in Langerhans cells (Breel et al. 1987).
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Mouse lymph node stroma.
- Fusion Partners
- Spleen cells from immunized rats were fused with Sp2/0 myeloma cells.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
Applications of Dendritic/Interdigitating Cells antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/25|
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||C E P WB||1 ml|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||C E IF P WB||0.5 mg|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||F IF WB||0.1 mg|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||F||1 mg|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||C E P RE||1 mg|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||F||0.5 ml|
|Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)||STAR71D650||F IF||0.1 mg|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||F IF WB||0.1 mg|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||C E P||0.5 mg|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||F||0.5 ml|
Application Based External Images
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for Dendritic/Interdigitating Cells antibody
Breel, M. et al. (1987) Dendritic cells of the mouse recognized by two monoclonal antibodies.
Eur J Immunol. 17 (11): 1555-9.
Schröder, N.W. et al. (2008) Innate immune responses during respiratory tract infection with a bacterial pathogen induce allergic airway sensitization.
J Allergy Clin Immunol. 122: 595-602.e5.
Bobryshev, Y.V. et al. (2001) Evidence that dendritic cells infiltrate atherosclerotic lesions in apolipoprotein E-deficient mice.
Histol Histopathol. 16: 801-8.
Serafini, B. et al. (2000) Intracerebral recruitment and maturation of dendritic cells in the onset and progression of experimental autoimmune encephalomyelitis.
Am J Pathol. 157: 1991-2002.
Schulte, D.J. et al. (2009) Involvement of innate and adaptive immunity in a murine model of coronary arteritis mimicking Kawasaki disease.
J Immunol. 183: 5311-8.
Stichel, C.C. and Luebbert, H. (2007) Inflammatory processes in the aging mouse brain: participation of dendritic cells and T-cells.
Neurobiol Aging. 28: 1507-21.
Rezzani, R. et al (2003) Cyclosporine-A treatment inhibits the expression of metabotropic glutamate receptors in rat thymus.
Acta Histochem. 105: 81-7.
Naiki, Y. et al. (2008) TLR/MyD88 and LXRα Signaling Pathways Reciprocally Control Chlamydia Pneumoniae-Induced Acceleration of Atherosclerosis
J Immunol. 181: 7176-85.
Martins, V.C. et al. (2008) Ltbetar signaling does not regulate Aire-dependent transcripts in medullary thymic epithelial cells.
J Immunol. 181: 400-7.
Taraban, V.Y. et al. (2008) Invariant NKT cells promote CD8+ cytotoxic T cell responses by inducing CD70 expression on dendritic cells.
J Immunol. 180: 4615-20.
Shimada, A. et al. (2006) Translocation pathway of the intratracheally instilled ultrafine particles from the lung into the blood circulation in the mouse.
Toxicol Pathol. 34: 949-57.
Martino, M.C. et al. (2005) Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental shigellosis.
J Infect Dis. 192: 136-48.
Stepkowski, S.M. et al. (2006) Immature syngeneic dendritic cells potentiate tolerance to pancreatic islet allografts depleted of donor dendritic cells in microgravity culture condition.
Transplantation. 82: 1756-63.
Anderson, K.L. et al. (2000) Transcription factor PU.1 is necessary for development of thymic and myeloid progenitor-derived dendritic cells.
J Immunol. 164: 1855-61.
Columba-Cabezas, S. et al. (2003) Lymphoid chemokines CCL19 and CCL21 are expressed in the central nervous system during experimental autoimmune encephalomyelitis: implications for the maintenance of chronic neuroinflammation.
Brain Pathol. 13: 38-51.
Sorrentino, R. et al. (2015) A single infection with Chlamydia pneumoniae is sufficient to exacerbate atherosclerosis in ApoE deficient mice.
Cell Immunol. 294: 25-32.
Zhao, X. et al. (2012) A combination of secondhand cigarette smoke and Chlamydia pneumoniae accelerates atherosclerosis.
Atherosclerosis. 222: 59-66.
Takakura I et al. (2011) Orally administered prion protein is incorporated by m cells and spreads into lymphoid tissues with macrophages in prion protein knockout mice.
Am J Pathol. 179 (3): 1301-9.
Fluorescent SpectraviewerWatch the Tool Tutorial Video ▸
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra