CD9 antibody | MF1

Rat anti Mouse CD9:Alexa Fluor®488

Product Type
Monoclonal Antibody
Clone
MF1
Isotype
IgG2b
Specificity
CD9

Product Code Applications Pack Size List Price Your Price Qty
MCA2749A488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Rat anti Mouse CD9 antibody, clone MF1 recognizes mouse CD9, a ~25 kDa member of the tetraspanin family of transmembrane proteins. The tetraspanin family are multifunctional molecules which bind to a wide range of transmembrane proteins and intracellular signalling molecules. Tetraspanins facilitate the formation of molecular complexes which play a role in cell activation, proliferation, motility, fusion and apoptosis.

CD9 is ubiquitously expressed, with high levels found on platelets and in male reproductive tissues. In mouse haematopoietic tissues, CD9 is expressed by myeloid and stromal cells. CD9 is known to complex with integrins and other transmembrane proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A or BUF041B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD9 antibody

  1. Etzerodt, A. et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma.
    Sci Rep. 7: 40286.

RRID
AB_10895973
UniProt
P40240
Entrez Gene
Cd9
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0008285 negative regulation of cell proliferation
GO:0030913 paranodal junction assembly

MCA2749A488

151732

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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