CD83 antibody | Michel-19
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Rat anti Mouse CD83
- Product Type
- Monoclonal Antibody
- Clone
- Michel-19
- Isotype
- IgG1
- Specificity
- CD83
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD83, clone Michel-19 recognizes CD83 also known as HB15. CD83 is a 45 kDa type I transmembrane protein and member of the immunoglobulin superfamily. CD83 expression has been detected on dendritic cells (DCs) and activated lymphocytes. CD83 is a well-recognized marker for mature dendritic cells. It is expressed as a cell surface molecule on thymic, circulating, and monocyte derived DCs, as well as Langerhans cells in the skin, interdigitating reticulum cells in the T cell zones of lymphoid organs, and microglia. Low level expression has also been reported on activated T and B cells. In addition, CD83 can be expressed in a soluble form, and is able to down-regulate DC maturation and stimulation of T cells. When CD83 expression was disrupted in mice, CD4+ T cell generation was impaired. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant mouse CD83 protein
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 | 1/80 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
References for CD83 antibody
-
Breloer, M. et al. (2007) CD83 is a regulator of murine B cell function in vivo.
Eur J Immunol. 37 (3): 634-48. -
Kretschmer, B. et al. (2009) Engagement of CD83 on B cells modulates B cell function in vivo.
J Immunol. 182 (5): 2827-34.
- UniProt
- O88324
- Entrez Gene
- Cd83
- GO Terms
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
- GO:0014070 response to organic cyclic compound
- GO:0032713 negative regulation of interleukin-4 production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032743 positive regulation of interleukin-2 production
- GO:0043372 positive regulation of CD4-positive, alpha beta T cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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