CD83 antibody | Michel-19

Rat anti Mouse CD83

Product Type
Monoclonal Antibody
Clone
Michel-19
Isotype
IgG1
Specificity
CD83

Product Code Applications Pack Size List Price Your Price Qty
MCA6064GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD83, clone Michel-19 recognizes CD83 also known as HB15. CD83 is a 45 kDa type I transmembrane protein and member of the immunoglobulin superfamily. CD83 expression has been detected on dendritic cells (DCs) and activated lymphocytes. CD83 is a well-recognized marker for mature dendritic cells. It is expressed as a cell surface molecule on thymic, circulating, and monocyte derived DCs, as well as Langerhans cells in the skin, interdigitating reticulum cells in the T cell zones of lymphoid organs, and microglia. Low level expression has also been reported on activated T and B cells. In addition, CD83 can be expressed in a soluble form, and is able to down-regulate DC maturation and stimulation of T cells. When CD83 expression was disrupted in mice, CD4+ T cell generation was impaired.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant mouse CD83 protein
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/80
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

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References for CD83 antibody

  1. Breloer, M. et al. (2007) CD83 is a regulator of murine B cell function in vivo.
    Eur J Immunol. 37 (3): 634-48.
  2. Kretschmer, B. et al. (2009) Engagement of CD83 on B cells modulates B cell function in vivo.
    J Immunol. 182 (5): 2827-34.

UniProt
O88324
Entrez Gene
Cd83
GO Terms
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
GO:0014070 response to organic cyclic compound
GO:0032713 negative regulation of interleukin-4 production
GO:0032733 positive regulation of interleukin-10 production
GO:0032743 positive regulation of interleukin-2 production
GO:0043372 positive regulation of CD4-positive, alpha beta T cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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