CD8 Alpha antibody | 53-6.7
Rat anti Mouse CD8α antibody, clone 53-6.7 (MCA2694) used to stain CD8 expressing tissue in mouse pancreatic sections by immunofluorescence.
Image caption:
Determination of Kb/B22–29-tetramer+ CD8 T-cells in diabetic RIP-B7.1 tg mice.
(C) The numbers of Kb/B22–29-tetramer+ CD8 T-cells were determined during the course of pCI/ppinsΔA12–21-mediated EAD: group 1, health mice (n = 3) with blood glucose levels <200 mg/dl; group 2, early diabetic mice (n = 3) with blood glucose levels between 250-350 mg/dl; group 3, diabetic mice (n = 3) with severe diabetes (i.e., blood glucose levels between 400-550 mg/dl). Pancreata of representative mice out of groups 1 to 3 were analyzed histologically for CD8 T-cell influx (CD8+) or stained with hematoxylin-eosin (H&E).
From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013)
A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner.
PLoS ONE 8(8): e71746.
Rat anti mouse CD8α antibody, clone 53-6.7 (MCA2694) used for the identification of CD8 expressing cells in the pancreata of normal and early diabetic mice by immunofluorescence
Image caption:
Ppins/(Kb/A12–21)-mediated recruitment of autoreactive T-cells into the pancreatic target tissue. PD-L1−/− mice were immunized with pCI/ppinsΔA12–21 (A) or with both, pCI/ppins+pCI/ppinsΔA12–21 vectors into the right and the left tibialis anterior muscles, respectively (B). Pancreata of representative healthy (A) and early diabetic mice (B) were analyzed histologically for insulin expression (insulin) and influx of CD8+ T-cells (CD8+), or stained with hematoxylin-eosin (H&E).
From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013)
A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner.
PLoS ONE 8(8): e71746.
Rat anti Mouse CD8α antibody, clone 53-6.7 (MCA2694) used to label CD8 expressing T cells in mouse brain by immunofluorescence.
Image caption:
Adoptive transfer of CD8+ cells successfully migrate to brain post induction of PIFS.
(A) Schematic illustrating the adoptive transfer of sorted perforin competent CD8+ cells into perforin−/− mice followed by induction of PIFS. C57BL/6 perforin−/− mice were irradiated with 400 rads of irradiation and then intravenously injected with 107 GFP+ CD8+ splenocytes (n = 10) or 107 Ly5.1+ CD8+ splenocytes (n = 10). Mice were intracranially infected with TMEV on the following day. PIFS-inducing VP2121–130 peptide or mock E7 control peptide were intravenously administered on day 7, during the peak of CD8 T cell expansion. MRI analysis was performed on the following day to visualize the extent of CNS vascular permeability and then the CNS was harvested for additional assays. Both negative (Experiment I) and positive (Experiment II) sort experiments yielded high purity of CD8+ T cell transfer. (B) Representative confocal microscopic images illustrating co-localization of Ly5.1 and CD8 protein (Experiment I). CNS-infiltrating Ly5.1+ cells colocalized with CD8+ cells 85.7% of the time as measured by confocal analysis. (C) Confocal microscopy showing of representative brain tissue slice showing successful transfer of GFP+ CD8+ T cells (Experiment II). Purity of transfer was analyzed via flow cytometry, 98.0% ± 0.5% of cells that were CD8 positive and GFP positive.
From: Johnson HL, Willenbring RC, Jin F, Manhart WA, LaFrance SJ, Pirko I, et al. (2014)
Perforin Competent CD8 T Cells Are Sufficient to Cause Immune-Mediated Blood-Brain Barrier Disruption.
PLoS ONE 9(10): e111401.
Rat anti Mouse CD8 Alpha
- Product Type
- Monoclonal Antibody
- Clone
- 53-6.7
- Isotype
- IgG2a
- Specificity
- CD8 Alpha
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2. CD8α is the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has also been used for depletion of CD8a+ cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse thymus or spleen.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat Pack Size: 25 µg 1/20 Pack Size: 0.25 mg |
1/20 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Immunohistology
- MCA2694 has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD8 Alpha antibody
-
Takahashi, K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells.
Proc Natl Acad Sci U S A. 89 (12): 5557-61. -
Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
J Mol Biol. 384 (5): 1190-202. -
McDole, J.R. et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33. -
Schuster, C. et al. (2013) A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.
PLoS One. 8 (8): e71746. -
Johnson, H.L. et al. (2014) Perforin competent CD8 T cells are sufficient to cause immune-mediated blood-brain barrier disruption.
PLoS One. 9 (10): e111401. -
Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
J Clin Invest. 125 (11): 4281-94.
Further Reading
-
Jabbari, A. & Harty, J.T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses.
J Leukoc Biol. 80 (1): 16-23.
- Synonyms
- LY-2
- RRID
- AB_905993
- UniProt
- P01731
- Entrez Gene
- Cd8a
- GO Terms
- GO:0002456 T cell mediated immunity
- GO:0016021 integral to membrane
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042803 protein homodimerization activity
- GO:0045065 cytotoxic T cell differentiation
- GO:0050850 positive regulation of calcium-mediated signaling
MCA2694T
149154
167596
MCA2694
150061
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