CD73 antibody | TY/11.8
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Rat anti Mouse CD73
- Product Type
- Monoclonal Antibody
- Clone
- TY/11.8
- Isotype
- IgG1
- Specificity
- CD73
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD73 antibody, clone TY/11.8 recognizes CD73 also known as ecto-5′-nucleotidase. CD73 is a ~69 kDa GPI anchored surface protein. CD73, in combination with CD39, catalyzes the conversion of purine 5′ mononucleotides to nucleosides. CD73 was initially defined as a marker of regulatory T cells (Schuler et al. 2011). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- CHO cells transfected with mouse CD73
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/80 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
References for CD73 antibody
-
Yoo, H.S. et al. (2013) Mesenchymal Stem Cell Lines Isolated by Different Isolation Methods Show Variations in the Regulation of Graft-versus-host Disease.
Immune Netw. 13 (4): 133-40.
Further Reading
-
Schuler PJ et al. (2011) Separation of human CD4+CD39+ T cells by magnetic beads reveals two phenotypically and functionally different subsets.
J Immunol Methods. 369 (1-2): 59-68.
- UniProt
- Q61503
- Entrez Gene
- Nt5e
- GO Terms
- GO:0005886 plasma membrane
- GO:0000166 nucleotide binding
- GO:0005624 membrane fraction
- GO:0006196 AMP catabolic process
- GO:0008253 5'-nucleotidase activity
- GO:0031225 anchored to membrane
- GO:0046086 adenosine biosynthetic process
- GO:0050728 negative regulation of inflammatory response
View more products with CD73 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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