CD71 antibody | ER-MP21

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Rat anti Mouse CD71:Low Endotoxin

Product Type
Monoclonal Antibody
Clone
ER-MP21
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2396EL F FN IP 0.5 mg
Rat anti Mouse CD71 antibody, clone ER-MP21 recognizes murine CD71, a ~200 kDa antigen, otherwise known as the transferrin receptor. CD71 is expressed by all proliferating cell populations and exists as a homodimer at the cell surface.

Rat anti Mouse CD71 antibody, clone ER-MP21 inhibits iron uptake and cell proliferation but does not compete with transferrin for binding to its receptor, which suggests that clone ER-MP21 binds to an epitope that is different from the transferrin-binding site (Leenen et al. 1990).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Balb/c macrophage precursor cell hybrids.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q62351 Related reagents
Entrez Gene
Tfrc Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0008233 peptidase activity
GO:0005381 iron ion transmembrane transporter activity
GO:0016021 integral to membrane
GO:0004998 transferrin receptor activity
GO:0005768 endosome
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0042470 melanosome
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular vesicular exosome
Regulatory
For research purposes only

Applications of CD71 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL 0.5 mg F

Product Specific References

References for CD71 antibody

  1. Leenen, P.J.M. et al. (1990) Differential inhibition of macrophage proliferation by anti-transferrin receptor antibody ER-MP21: correlation to macrophage differentiation stage.
    Exp Cell Res.189:55 – 63.
  2. Moos, T. (1995) Increased accumulation of transferrin by motor neurons of the mouse mutant progressive motor neuronopathy (pmn/pmn).
    J Neurocytol. 1995 May;24(5):389-98.
  3. Brekelmans, P. et al. (1994) Inhibition of proliferation and differentiation during early T cell development by anti-transferrin receptor antibody.
    Eur J Immunol. 24 (11): 2896-902.

Applications

These CD71 antibodies can be used for applications including:


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