Rat anti Mouse CD71 antibody, clone ER-MP21
recognizes murine CD71, a ~200 kDa antigen, otherwise known as the transferrin receptor. CD71 is expressed by all proliferating cell populations and exists as a homodimer at the cell surface.
Rat anti Mouse CD71 antibody, clone ER-MP21 inhibits iron uptake and cell proliferation but does not compete with transferrin for binding to its receptor, which suggests that clone ER-MP21 binds to an epitope that is different from the transferrin-binding site (Leenen et al. 1990
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Balb/c macrophage precursor cell hybrids.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
iron ion transmembrane transporter activity
integral to membrane
transferrin receptor activity
cellular iron ion homeostasis
perinuclear region of cytoplasm
extracellular vesicular exosome
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD71 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD71 antibody
Leenen, P.J.M. et al. (1990) Differential inhibition of macrophage proliferation by anti-transferrin receptor antibody ER-MP21: correlation to macrophage differentiation stage.
Exp Cell Res.189:55 – 63.
Moos, T. (1995) Increased accumulation of transferrin by motor neurons of the mouse mutant progressive motor neuronopathy (pmn/pmn).
J Neurocytol. 1995 May;24(5):389-98.
Brekelmans, P. et al. (1994) Inhibition of proliferation and differentiation during early T cell development by anti-transferrin receptor antibody.
Eur J Immunol. 24 (11): 2896-902.