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CD70 antibody | FR70

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Anti Mouse CD70 Antibody, clone FR70 thumbnail image 1 Anti Mouse CD70 Antibody, clone FR70 thumbnail image 2 Anti Mouse CD70 Antibody, clone FR70 thumbnail image 3 Anti Mouse CD70 Antibody, clone FR70 thumbnail image 4
Anti Mouse CD70 Antibody, clone FR70 gallery image 1
Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70 (MCA2302)
Anti Mouse CD70 Antibody, clone FR70 gallery image 2
Staining of LPS activated mouse spleen cells with Alexa Fluor® 647 conjugated Rat anti Mouse CD70: (MCA2302A647)
Anti Mouse CD70 Antibody, clone FR70 gallery image 3
Staining of LPS activated mouse spleen cells with Alexa Fluor® 488 conjugated Rat anti Mouse CD70 (MCA2302A488)
Anti Mouse CD70 Antibody, clone FR70 gallery image 4
Staining of LPS stimulated mouse spleen cells with biotinylated Rat anti-Mouse CD70 (MCA2302B)

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Rat anti Mouse CD70:RPE

Product Type
Monoclonal Antibody
Clone
FR70
Isotype
IgG2b
Specificity
CD70

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Product Code Applications Pack Size List Price Your Price Qty
MCA2302PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse CD70 antibody, clone FR70 recognizes mouse CD70, a ~30-33 kDa single pass type II transmembrane glycoprotein belonging to the TNF superfamily. CD70 is not expressed on resting T cells and B cells but expression is induced on activation. Interaction of CD70 with its ligand CD27 is reported to play an important role in T cell co-stimulation and the regulation of T-dependant B cell activation and antibody production (Nolte et al. 2009).

Rat anti Mouse CD70 antibody, clone FR70 blocks functionality of CD70 and has been used to block the interactions between CD27 and CD70 (Oshima et al.).

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
A20 mouse B cell lymphoma cell line
Fusion Partners
Spleen cells from immunized F344/DuCrj rats were fused with cells of the mouse P3U1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD70 antibody

  1. Oshima, H. et al. (1998) Characterization of murine CD70 by molecular cloning and mAb.
    Int Immunol. 10 (4): 517-26.
  2. Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
    J Immunol. 164 (4): 1741-5.
  3. Akiba, H. et al. (2000) Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis.
    J Exp Med. 191 (2): 375-80.
  4. Akiba, H. et al. (1999) CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells.
    J Immunol. 162 (12): 7058-66.

Flow Cytometry

Functional Assays

Synonyms
TNFSF7
UniProt
O55237
Entrez Gene
Cd70
GO Terms
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response

MCA2302PE

INN1701

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