CD70 antibody | FR70
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F ResetRat anti Mouse CD70:RPE
- Product Type
- Monoclonal Antibody
- Clone
- FR70
- Isotype
- IgG2b
- Specificity
- CD70
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD70 antibody, clone FR70 recognizes mouse CD70, a ~30-33 kDa single pass type II transmembrane glycoprotein belonging to the TNF superfamily. CD70 is not expressed on resting T cells and B cells but expression is induced on activation. Interaction of CD70 with its ligand CD27 is reported to play an important role in T cell co-stimulation and the regulation of T-dependant B cell activation and antibody production (Nolte et al. 2009). Rat anti Mouse CD70 antibody, clone FR70 blocks functionality of CD70 and has been used to block the interactions between CD27 and CD70 (Oshima et al.). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- A20 mouse B cell lymphoma cell line
- Fusion Partners
- Spleen cells from immunized F344/DuCrj rats were fused with cells of the mouse P3U1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse Seroblock FcR | ||||||
References for CD70 antibody
-
Oshima, H. et al. (1998) Characterization of murine CD70 by molecular cloning and mAb.
Int Immunol. 10 (4): 517-26. -
Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
J Immunol. 164 (4): 1741-5. -
Akiba, H. et al. (2000) Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis.
J Exp Med. 191 (2): 375-80. -
Akiba, H. et al. (1999) CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells.
J Immunol. 162 (12): 7058-66.
- Synonyms
- TNFSF7
- UniProt
- O55237
- Entrez Gene
- Cd70
- GO Terms
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
View more products with CD70 specificity
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