CD70 antibody | FR70




Rat anti Mouse CD70:Alexa Fluor® 488
Rat anti Mouse CD70:Alexa Fluor® 647
Rat anti Mouse CD70:RPE
- Product Type
- Monoclonal Antibody
- Clone
- FR70
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2302A488 | F | 100 Tests/1ml |
![]() |
|
MCA2302A647 | F | 100 Tests/1ml |
![]() |
|
MCA2302PE | F | 100 Tests |
![]() |
Rat anti Mouse CD70 antibody, clone FR70 blocks functionality of CD70 and has been used to block the interactions between CD27 and CD70 (Oshima et al.).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- A20 mouse B cell lymphoma cell line
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized F344/DuCrj rats were fused with cells of the mouse P3U1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O55237
- Entrez Gene
- Cd70
- GO Terms
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD70 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
![]() |
|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
![]() |
|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
![]() |
|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
![]() |
|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
![]() |
|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
![]() |
Product Specific References
References for CD70 antibody
-
Oshima, H. et al. (1998) Characterization of murine CD70 by molecular cloning and mAb.
Int Immunol. 10 (4): 517-26. -
Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
J Immunol. 164 (4): 1741-5. -
Akiba, H. et al. (2000) Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis.
J Exp Med. 191 (2): 375-80. -
Akiba, H. et al. (1999) CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells.
J Immunol. 162 (12): 7058-66.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra