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CD54 antibody | 3E2B

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Anti Mouse CD54 Antibody, clone 3E2B gallery image 1
Published customer image:
Hamster anti Mouse CD54 antibody, clone 3E2B (MCA1371Z) used for the detection of Mouse ICAM-1 in murine liver cryosections by immunohistochemistry.
Image caption:
Expression of lymphoid structure markers in livers of aly BM-chimeric mice. Liver cryosections from aly BM-chimeras immunized s.c. with MOG35–55 (d11) were stained with antibodies against CD4, CD8, CD11b, CD11c, CD19, CD62L, CD68, FDC, ICAM, Ki67, PNA, and VCAM. Bar indicates 200 μm.

From: Greter M, Hofmann J, Becher B (2009)
Neo-Lymphoid Aggregates in the Adult Liver Can Initiate Potent Cell-Mediated Immunity.
PLoS Biol 7(5): e1000109.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Hamster anti Mouse CD54:Preservative Free

Product Type
Monoclonal Antibody
Clone
3E2B
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA1371Z
Datasheet
C E F FN
SDS
0.5 mg loader

Hamster anti Mouse CD54 antibody, clone 3E2B recognizes murine Intercellular adhesion molecule 1, also known as ICAM-1, CD54, MALA-2 or MyD10. CD54 is a 537 amino acid ~89kDa single pass type I transmembrane glycoprotein bearing five Ig-like C2 type domains. Two isoforms, differing at the N-terminal signal peptide sequence and flanking end terminal domain of the processed molecule are generated by alternative splicing (UniProt P13577). CD45 is a counter-receptor for LFA-1 (Diamond et al. 1990).

Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.

Sterile filtered.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P13597
Entrez Gene
Icam1
GO Terms
GO:0001772 immunological synapse
GO:0002457 T cell antigen processing and presentation
GO:0016021 integral to membrane
GO:0007159 leukocyte cell-cell adhesion
GO:0009897 external side of plasma membrane
GO:0030155 regulation of cell adhesion
GO:0033627 cell adhesion mediated by integrin
Regulatory
For research purposes only

Applications of CD54 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry neat
Functional Assays
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD54 antibody

  1. Scheynius, A. et al. (1993) Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
    J Immunol. 150 (2): 655-63.
  2. Fabry, Z. et al. (1992) Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
    J Neuroimmunol. 36 (1): 1-11.
  3. Greter, M. et al. (2009) Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity.
    PLoS Biol. 7 (5): e1000109.
  4. Brochard, V. et al. (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
    J Clin Invest. 119: 182-92.
  5. Stein, S. et al. (2010) SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice.
    Aging (Albany NY). 2: 353-60.
  6. Impellizzeri, D. et al. (2015) The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease.
    Clin Nutr. 34 (6): 1146-54.
  7. Cherry, J.D. et al. (2013) Thermal injury lowers the threshold for radiation-induced neuroinflammation and cognitive dysfunction.
    Radiat Res. 180 (4): 398-406.
  8. Fusco, R.et al. (2017) A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease
    J Funct Foods. 30: 168-78.
  9. Xuemei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation[Preprint].
    Nov 13. [Epub ahead of print].

Further Reading

  1. Johnston, S.C. et al. (1990) On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
    J Immunol. 145 (4): 1181-7.

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