CD54 antibody | 3E2B
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
Sterile filtered. - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P13597
- Entrez Gene
- Icam1
- GO Terms
- GO:0001772 immunological synapse
- GO:0002457 T cell antigen processing and presentation
- GO:0016021 integral to membrane
- GO:0007159 leukocyte cell-cell adhesion
- GO:0009897 external side of plasma membrane
- GO:0030155 regulation of cell adhesion
- GO:0033627 cell adhesion mediated by integrin
- Regulatory
- For research purposes only
Applications of CD54 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | neat | ||
Functional Assays | |||
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
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|
Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
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|
Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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Product Specific References
References for CD54 antibody
-
Scheynius, A. et al. (1993) Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
J Immunol. 150 (2): 655-63. -
Fabry, Z. et al. (1992) Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
J Neuroimmunol. 36 (1): 1-11. -
Greter, M. et al. (2009) Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity.
PLoS Biol. 7 (5): e1000109. -
Brochard, V. et al. (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
J Clin Invest. 119: 182-92. -
Stein, S. et al. (2010) SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice.
Aging (Albany NY). 2: 353-60. -
Impellizzeri, D. et al. (2015) The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease.
Clin Nutr. 34 (6): 1146-54. -
Cherry, J.D. et al. (2013) Thermal injury lowers the threshold for radiation-induced neuroinflammation and cognitive dysfunction.
Radiat Res. 180 (4): 398-406. -
Fusco, R.et al. (2017) A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease
J Funct Foods. 30: 168-78. -
Xuemei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation[Preprint].
Nov 13. [Epub ahead of print].
Further Reading
-
Johnston, S.C. et al. (1990) On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
J Immunol. 145 (4): 1181-7.
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