Hamster anti Mouse CD54 antibody, clone 3E2B
recognizes murine Intercellular adhesion molecule 1, also known as ICAM-1, CD54, MALA-2 or MyD10. CD54 is a 537 amino acid ~89kDa single pass type I transmembrane glycoprotein bearing five Ig-like C2 type domains. Two isoforms, differing at the N-terminal signal peptide sequence and flanking end terminal domain of the processed molecule are generated by alternative splicing (UniProt P13577
). CD45 is a counter-receptor for LFA-1
(Diamond et al. 1990
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
T cell antigen processing and presentation
integral to membrane
leukocyte cell-cell adhesion
external side of plasma membrane
regulation of cell adhesion
cell adhesion mediated by integrin
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD54 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for CD54 antibody
Scheynius, A. et al. (1993) Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
J Immunol. 150 (2): 655-63.
Fabry, Z. et al. (1992) Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
J Neuroimmunol. 36 (1): 1-11.
Greter, M. et al. (2009) Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity.
PLoS Biol. 7 (5): e1000109.
Brochard, V. et al. (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
J Clin Invest. 119: 182-92.
Stein, S. et al. (2010) SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice.
Aging (Albany NY). 2: 353-60.
Impellizzeri, D. et al. (2015) The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease.
Clin Nutr. 34 (6): 1146-54.
Cherry, J.D. et al. (2013) Thermal injury lowers the threshold for radiation-induced neuroinflammation and cognitive dysfunction.
Radiat Res. 180 (4): 398-406.
Fusco, R.et al. (2017) A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease
J Funct Foods. 30: 168-78.
Xuemei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation[Preprint].
Nov 13. [Epub ahead of print].
Johnston, S.C. et al. (1990) On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
J Immunol. 145 (4): 1181-7.
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