CD54 antibody | 3E2B
Hamster anti Mouse CD54 antibody, clone 3E2B used for the detection of Mouse ICAM-1 in murine liver cryosections by immunohistochemistry.
Image caption:
Expression of lymphoid structure markers in livers of aly BM-chimeric mice. Liver cryosections from aly BM-chimeras immunized s.c. with MOG35–55 (d11) were stained with antibodies against CD4, CD8, CD11b, CD11c, CD19, CD62L, CD68, FDC, ICAM, Ki67, PNA, and VCAM. Bar indicates 200 μm.
From: Greter M, Hofmann J, Becher B (2009)
Neo-Lymphoid Aggregates in the Adult Liver Can Initiate Potent Cell-Mediated Immunity.
PLoS Biol 7(5): e1000109.
Hamster anti Mouse CD54 antibody, clone 3E2B recognizes murine Intercellular adhesion molecule 1, also known as ICAM-1, CD54, MALA-2 or MyD10. CD54 is a 537 amino acid ~89kDa single pass type I transmembrane glycoprotein bearing five Ig-like C2 type domains. Two isoforms, differing at the N-terminal signal peptide sequence and flanking end terminal domain of the processed molecule are generrated by alternative splicing(UniProt P13577) . CD45 is a counter-receptor for LFA-1 (Diamond et al. 1990)
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
Sterile filtered. - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Please see label for expiry date.
More Information
- UniProt
- P13597 Related reagents
- Entrez Gene
- Icam1 Related reagents
- GO Terms
- GO:0001772 immunological synapse
- GO:0002457 T cell antigen processing and presentation
- GO:0016021 integral to membrane
- GO:0007159 leukocyte cell-cell adhesion
- GO:0009897 external side of plasma membrane
- GO:0030155 regulation of cell adhesion
- GO:0033627 cell adhesion mediated by integrin
- Regulatory
- For research purposes only
Applications of CD54 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | neat | ||
| Functional Assays | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:DyLight®549 | STAR104D549GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:DyLight®649 | STAR104D649GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | 0.1 mg | F IF WB | ||
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | 0.4 mg | F | ||
| Goat anti Hamster IgG:Biotin | STAR79B | 0.8 mg | E F | ||
| Goat anti Hamster IgG:FITC | STAR79F | 0.7 mg | F | ||
| Goat anti Hamster IgG:HRP | STAR79P | 0.8 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | 0.25 mg | F |
Product Specific References
References for CD54 antibody
-
Scheynius, A. et al. (1993) Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
J Immunol. 150 (2): 655-63. -
Fabry, Z. et al. (1992) Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
J Neuroimmunol. 36 (1): 1-11. -
Johnston, S.C. et al. (1990) On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
J Immunol. 145 (4): 1181-7. -
Greter, M. et al. (2009) Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity.
PLoS Biol. 7 (5): e1000109. -
Brochard, V. et al. (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
J Clin Invest. 119: 182-92. -
Stein, S. et al. (2010) SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice.
Aging (Albany NY). 2: 353-60. -
Impellizzeri, D. et al. (2015) The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease.
Clin Nutr. 34 (6): 1146-54. -
Cherry, J.D. et al. (2013) Thermal injury lowers the threshold for radiation-induced neuroinflammation and cognitive dysfunction.
Radiat Res. 180 (4): 398-406. -
Fusco, R.et al. (2017) A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease
J Funct Foods. 30: 168-78.
