CD54 antibody | 3E2B
Hamster anti Mouse CD54 antibody, clone 3E2B (MCA1371Z) used for the detection of Mouse ICAM-1 in murine liver cryosections by immunohistochemistry.
Image caption:
Expression of lymphoid structure markers in livers of aly BM-chimeric mice. Liver cryosections from aly BM-chimeras immunized s.c. with MOG35–55 (d11) were stained with antibodies against CD4, CD8, CD11b, CD11c, CD19, CD62L, CD68, FDC, ICAM, Ki67, PNA, and VCAM. Bar indicates 200 μm.
From: Greter M, Hofmann J, Becher B (2009)
Neo-Lymphoid Aggregates in the Adult Liver Can Initiate Potent Cell-Mediated Immunity.
PLoS Biol 7(5): e1000109.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD54 antibody, clone 3E2B recognizes murine Intercellular adhesion molecule 1, also known as ICAM-1, CD54, MALA-2 or MyD10. CD54 is a 537 amino acid ~89kDa single pass type I transmembrane glycoprotein bearing five Ig-like C2 type domains. Two isoforms, differing at the N-terminal signal peptide sequence and flanking end terminal domain of the processed molecule are generated by alternative splicing (UniProt P13577). CD45 is a counter-receptor for LFA-1 (Diamond et al. 1990).
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
Sterile filtered. - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P13597
- Entrez Gene
- Icam1
- GO Terms
- GO:0001772 immunological synapse
- GO:0002457 T cell antigen processing and presentation
- GO:0016021 integral to membrane
- GO:0007159 leukocyte cell-cell adhesion
- GO:0009897 external side of plasma membrane
- GO:0030155 regulation of cell adhesion
- GO:0033627 cell adhesion mediated by integrin
- Regulatory
- For research purposes only
Applications of CD54 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | neat | ||
| Functional Assays | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
|
|
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
|
|
| Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
|
|
| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
|
|
| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
|
Product Specific References
References for CD54 antibody
-
Scheynius, A. et al. (1993) Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
J Immunol. 150 (2): 655-63. -
Fabry, Z. et al. (1992) Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
J Neuroimmunol. 36 (1): 1-11. -
Greter, M. et al. (2009) Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity.
PLoS Biol. 7 (5): e1000109. -
Brochard, V. et al. (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
J Clin Invest. 119: 182-92. -
Stein, S. et al. (2010) SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice.
Aging (Albany NY). 2: 353-60. -
Impellizzeri, D. et al. (2015) The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease.
Clin Nutr. 34 (6): 1146-54. -
Cherry, J.D. et al. (2013) Thermal injury lowers the threshold for radiation-induced neuroinflammation and cognitive dysfunction.
Radiat Res. 180 (4): 398-406. -
Fusco, R.et al. (2017) A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease
J Funct Foods. 30: 168-78.
Further Reading
-
Johnston, S.C. et al. (1990) On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
J Immunol. 145 (4): 1181-7.
