CD5 antibody | YTS 121.5.2

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Rat anti Mouse CD5:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
YTS 121.5.2
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2280A647T F 25 Tests/0.25ml
MCA2280A647 F 100 Tests/1ml
Rat anti Mouse CD5 antibody, clone YTS 121.5.2 recognizes murine CD5 (Lyt-1) a cell surface glycoprotein expressed by T-lymphocytes.
Studies suggest that CD5 plays an important role in the regulation of immune tolerance and lymphocyte selection.
Rat anti Mouse CD5 antibody, clone YTS 121.5.2 is suitable for T-cell depletion in vivo (Cobbold et al. 1984).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
CBA mouse thymocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised DA Rats were fused with cells of the rat Y3Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13379 Related reagents
Entrez Gene
Cd5 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD5 antibody

  1. Cobbold, S.P. et al. (1984) Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo.
    Nature. 312 (5994): 548-51.
  2. Michel, B. et al. (2012) Incunabular immunological events in prion trafficking.
    Sci Rep. 2: 440.