CD5 antibody | 53-7.3

Rat anti Mouse CD5

Product Type
Monoclonal Antibody
Clone
53-7.3
Isotype
IgG2a
Specificity
CD5

Product Code Applications Pack Size List Price Your Price Qty
MCA6052GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD5 recognizes the CD5 cell surface glycoprotein. CD5 is a 67 kDa member of the scavenger receptor cysteine-rich protein superfamily. CD5 is primarily expressed on an IgM subset of B cells and alpha/beta T cells showing very little expression on gamma delta T cells. CD5 is also expressed by tumor cells in cases of B Cell Chronic Lymphocytic Leukaemia (B-CLL). The role for CD5 involves its interactions with antigen receptors on both T cells and B cells although the precise function has not been elucidated. The ability of CD5 to inhibit TCR signaling implies that it is important in T cell development and activation.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse thymus or spleen
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
List Price Your Price
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Description Rat IgG2a Negative Control

UniProt
P06127
Entrez Gene
CD5
GO Terms
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
GO:0008037 cell recognition
GO:0008283 cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products