CD49d antibody | 9C10 (MFR4.B)

Rat anti Mouse CD49d

Product Type
Monoclonal Antibody
Clone
9C10 (MFR4.B)
Isotype
IgG2a
Specificity
CD49d

Product Code Applications Pack Size List Price Your Price Qty
MCA6063GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD49d, clone 9C10 recognizes CD49d also known as alpha 4 integrin or VLA-4 alpha chain. A member of the integrin family, CD49d is expressed in combination with one of the two beta chains. It combines with CD29 to form integrin alpha 4 beta 1 or with integrin beta 7 to form alpha 4 beta 7. CD49d plays a role in both T cell stimulation and cellular adhesion.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
(C57BL/6 x A/J)F1 mouse fetal liver mass
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

Source Reference

  1. Kinashi, T. & Springer, T.A. (1994) Adhesion molecules in hematopoietic cells.
    Blood Cells. 20 (1): 25-44.

UniProt
Q00651
Entrez Gene
Itga4
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001968 fibronectin binding
GO:0001974 blood vessel remodeling
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007507 heart development
GO:0009897 external side of plasma membrane
GO:0016477 cell migration
GO:0060324 face development
GO:0060710 chorio-allantoic fusion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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