CD49d antibody | 9C10 (MFR4.B)

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Anti Mouse CD49d Antibody, clone 9C10 (MFR4.B) gallery image 1 — Staining of mouse peripheral blood lymphocytes with Alexa Fluor^® 647 Rat anti Mouse CD49d antibody, clone 9C10 (**MCA2294A647**).

Rat anti Mouse CD49d:Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: 9C10 (MFR4.B)
Isotype: IgG2a

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA2294A647 Datasheet	F SDS	100 Tests/1ml	Log in		Get Quote
MCA2294A647T Datasheet	F SDS	25 Tests/0.25ml	Log in		Get Quote

Rat anti Mouse CD49d monoclonal antibody, clone 9C10 recognizes murine alpha 4 integrin (CD49d) also known as VLA-4 subunit alpha, CD49d is a ~150 kDa single pass type I transmembrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1.

Clone 9C10 (MFR4.B) is reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2 (Kinashi and Springer 1994).

Product Details

Target Species

Mouse

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Mouse LyD9 haematopoietic progenitor cells.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion Partners

Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q00651
Entrez Gene: Itga4
GO Terms: GO:0007157 heterophilic cell-cell adhesion; GO:0001968 fibronectin binding; GO:0001974 blood vessel remodeling; GO:0004872 receptor activity; GO:0008305 integrin complex; GO:0007159 leukocyte cell-cell adhesion; GO:0007229 integrin-mediated signaling pathway; GO:0007507 heart development; GO:0009897 external side of plasma membrane; GO:0016477 cell migration; GO:0060324 face development; GO:0060710 chorio-allantoic fusion
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD49d antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647	MCA1212A647	F	100 Tests/1ml	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in		Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in		Get Quote

Product Specific References

Source Reference

Kinashi, T. et al. (1994) Adhesion Molecules in Hematopoietic Cells.
Blood Cells. 20: 25-44

References for CD49d antibody

Bellingan, G.J. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.
J Exp Med. 196 (11): 1515-21.

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