CD49d antibody | 9C10 (MFR4.B)

Rat anti Mouse CD49d:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
9C10 (MFR4.B)
Isotype
IgG2a
Specificity
CD49d

Product Code Applications Pack Size List Price Your Price Qty
MCA2294A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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MCA2294A647T
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SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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Rat anti Mouse CD49d monoclonal antibody, clone 9C10 recognizes murine alpha 4 integrin (CD49d) also known as VLA-4 subunit alpha, CD49d is a ~150 kDa single pass type I transmembrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1.

Clone 9C10 (MFR4.B) is reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2 (Kinashi and Springer 1994).

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse LyD9 haematopoietic progenitor cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
List Price Your Price
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Description Rat IgG2a Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

Source Reference

  1. Kinashi, T. et al. (1994) Adhesion Molecules in Hematopoietic Cells.
    Blood Cells. 20: 25-44

References for CD49d antibody

  1. Bellingan, G.J. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.
    J Exp Med. 196 (11): 1515-21.

Synonyms
Integrin Alpha 4 Chain
VLA-4
RRID
AB_566808
UniProt
Q00651
Entrez Gene
Itga4
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001968 fibronectin binding
GO:0001974 blood vessel remodeling
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007507 heart development
GO:0009897 external side of plasma membrane
GO:0016477 cell migration
GO:0060324 face development
GO:0060710 chorio-allantoic fusion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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