CD45RB antibody | C363-16A

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MCA4704
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C F IP P 0.25 mg loader
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Rat anti Mouse CD45RB antibody, clone C363-16A recognises an isoform of CD45 called CD45RB. CD45 is a member of the protein tyrosine phosphatase (PTP) family. The PTP proteins are signalling molecules that are involved in a variety of cellular processes including cell growth, differentiation, mitosis and oncogenesis. CD45 is an essential regulator of T- and B-cell antigen receptor signalling.

CD45 has various isoforms generated by different splicing of exons. Clone C363-16A recognises CD45RB, generated by exon 5 splicing. This 220kDa glycoprotein is expressed on peripheral B-cells, naïve T-cells, thymocytes, macrophages and dendritic cells.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Cloned mouse Th2 cell lines.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/50
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD45RB antibody

  1. Wu, Y. et al. (1997) CTLA-4-B7 interaction is sufficient to costimulate T cell clonal expansion.
    J Exp Med. 185:1327-35.
  2. Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
    J Exp Med. 169: 1179-84.

Further Reading

  1. Bottomly K. (1988) A functional dichotomy in CD4+ T lymphocytes.
    Immunol Today. 9:268-74. Review.

Flow Cytometry

RRID
AB_2269110
UniProt
P06800
Entrez Gene
Ptprc
GO Terms
GO:0000187 activation of MAPK activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0051726 regulation of cell cycle
GO:0004725 protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007159 leukocyte cell-cell adhesion
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0031953 negative regulation of protein autophosphorylation
GO:0034113 heterotypic cell-cell adhesion
GO:0042098 T cell proliferation
GO:0042100 B cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0043395 heparan sulfate proteoglycan binding
GO:0043410 positive regulation of MAPKKK cascade
GO:0045059 positive thymic T cell selection
GO:0045060 negative thymic T cell selection
GO:0045121 membrane raft
GO:0045577 regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050855 regulation of B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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