Rat anti Mouse CD45RB antibody, clone C363-16A recognises an isoform of CD45 called CD45RB. CD45 is a member of the protein tyrosine phosphatase (PTP) family. The PTP proteins are signalling molecules that are involved in a variety of cellular processes including cell growth, differentiation, mitosis and oncogenesis. CD45 is an essential regulator of T- and B-cell antigen receptor signalling.
CD45 has various isoforms generated by different splicing of exons. Clone C363-16A recognises CD45RB, generated by exon 5 splicing. This 220kDa glycoprotein is expressed on peripheral B-cells, naïve T-cells, thymocytes, macrophages and dendritic cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Cloned mouse Th2 cell lines.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
negative regulation of T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
positive regulation of humoral immune response mediated by circulating immunoglobulin
regulation of cell cycle
protein tyrosine phosphatase activity
integral to plasma membrane
negative regulation of protein kinase activity
leukocyte cell-cell adhesion
external side of plasma membrane
protein kinase binding
B cell differentiation
positive regulation of B cell proliferation
negative regulation of protein autophosphorylation
heterotypic cell-cell adhesion
T cell proliferation
B cell proliferation
positive regulation of apoptosis
heparan sulfate proteoglycan binding
positive regulation of MAPKKK cascade
positive thymic T cell selection
negative thymic T cell selection
regulation of B cell differentiation
positive regulation of gamma-delta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of peptidyl-tyrosine phosphorylation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45RB antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for CD45RB antibody
Wu, Y. et al. (1997) CTLA-4-B7 interaction is sufficient to costimulate T cell clonal expansion.
J Exp Med. 185:1327-35.
Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
J Exp Med. 169: 1179-84.
Bottomly K. (1988) A functional dichotomy in CD4+ T lymphocytes.
Immunol Today. 9:268-74. Review.
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