CD45RB antibody | C363-16A
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Rat anti Mouse CD45RB antibody, clone C363-16A recognises an isoform of CD45 called CD45RB. CD45 is a member of the protein tyrosine phosphatase (PTP) family. The PTP proteins are signalling molecules that are involved in a variety of cellular processes including cell growth, differentiation, mitosis and oncogenesis. CD45 is an essential regulator of T- and B-cell antigen receptor signalling.
CD45 has various isoforms generated by different splicing of exons. Clone C363-16A recognises CD45RB, generated by exon 5 splicing. This 220kDa glycoprotein is expressed on peripheral B-cells, naïve T-cells, thymocytes, macrophages and dendritic cells.
CD45 has various isoforms generated by different splicing of exons. Clone C363-16A recognises CD45RB, generated by exon 5 splicing. This 220kDa glycoprotein is expressed on peripheral B-cells, naïve T-cells, thymocytes, macrophages and dendritic cells.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Cloned mouse Th2 cell lines.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06800
- Entrez Gene
- Ptprc
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0051726 regulation of cell cycle
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007159 leukocyte cell-cell adhesion
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0031953 negative regulation of protein autophosphorylation
- GO:0034113 heterotypic cell-cell adhesion
- GO:0042098 T cell proliferation
- GO:0042100 B cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045059 positive thymic T cell selection
- GO:0045060 negative thymic T cell selection
- GO:0045121 membrane raft
- GO:0045577 regulation of B cell differentiation
- GO:0045588 positive regulation of gamma-delta T cell differentiation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050855 regulation of B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
- Regulatory
- For research purposes only
Applications of CD45RB antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/20 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for CD45RB antibody
-
Wu, Y. et al. (1997) CTLA-4-B7 interaction is sufficient to costimulate T cell clonal expansion.
J Exp Med. 185:1327-35. -
Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
J Exp Med. 169: 1179-84.
Further Reading
-
Bottomly K. (1988) A functional dichotomy in CD4+ T lymphocytes.
Immunol Today. 9:268-74. Review.
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