CD45RB antibody | C363-16A

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Rat anti Mouse CD45RB

Product Type: Monoclonal Antibody
Clone: C363-16A
Isotype: IgG2a

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA4704	C F IP P			0.25 mg

Rat anti Mouse CD45RB antibody, clone C363-16A recognises an isoform of CD45 called CD45RB. CD45 is a member of the protein tyrosine phosphatase (PTP) family. The PTP proteins are signalling molecules that are involved in a variety of cellular processes including cell growth, differentiation, mitosis and oncogenesis. CD45 is an essential regulator of T- and B-cell antigen receptor signalling.

CD45 has various isoforms generated by different splicing of exons. Clone C363-16A recognises CD45RB, generated by exon 5 splicing. This 220kDa glycoprotein is expressed on peripheral B-cells, naïve T-cells, thymocytes, macrophages and dendritic cells.

Product Details

Target Species: Mouse
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Cloned mouse Th2 cell lines.
Approx. Protein Concentrations: IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P06800 Related reagents
Entrez Gene: Ptprc Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0001915 negative regulation of T cell mediated cytotoxicity; GO:0001916 positive regulation of T cell mediated cytotoxicity; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin; GO:0051726 regulation of cell cycle; GO:0004725 protein tyrosine phosphatase activity; GO:0005887 integral to plasma membrane; GO:0005925 focal adhesion; GO:0006469 negative regulation of protein kinase activity; GO:0006470 protein dephosphorylation; GO:0007159 leukocyte cell-cell adhesion; GO:0008201 heparin binding; GO:0009897 external side of plasma membrane; GO:0019901 protein kinase binding; GO:0030183 B cell differentiation; GO:0030890 positive regulation of B cell proliferation; GO:0031953 negative regulation of protein autophosphorylation; GO:0034113 heterotypic cell-cell adhesion; GO:0042098 T cell proliferation; GO:0042100 B cell proliferation; GO:0043065 positive regulation of apoptosis; GO:0043395 heparan sulfate proteoglycan binding; GO:0043410 positive regulation of MAPKKK cascade; GO:0045059 positive thymic T cell selection; GO:0045060 negative thymic T cell selection; GO:0045121 membrane raft; GO:0045577 regulation of B cell differentiation; GO:0045588 positive regulation of gamma-delta T cell differentiation; GO:0046641 positive regulation of alpha-beta T cell proliferation; GO:0048304 positive regulation of isotype switching to IgG isotypes; GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0050855 regulation of B cell receptor signaling pathway; GO:0050857 positive regulation of antigen receptor-mediated signaling pathway; GO:0051209 release of sequestered calcium ion into cytosol; GO:0051607 defense response to virus
Regulatory: For research purposes only

Applications of CD45RB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/20	1/50
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	1 ml	C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	0.5 mg	C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	1 mg	F
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	1 mg	C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	0.5 ml	F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)	STAR71D649GA	0.1 mg	F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	0.1 mg	F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	0.5 mg	C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	0.5 ml	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rat IgG2a Negative Control	MCA1212	1 ml	E F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

Application Based External Images

Flow Cytometry

Product Specific References

References for CD45RB antibody

Wu, Y. et al. (1997) CTLA-4-B7 interaction is sufficient to costimulate T cell clonal expansion.
J Exp Med. 185:1327-35.
Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
J Exp Med. 169: 1179-84.