CD339 antibody | HMJ1-29

Hamster anti Mouse CD339

Product Type
Monoclonal Antibody
Clone
HMJ1-29
Isotype
IgG
Specificity
CD339

Product Code Applications Pack Size List Price Your Price Qty
MCA5707GA
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SDS Safety Datasheet SDS
C F 0.1 mg loader
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Hamster anti Mouse CD339 antibody, clone HMJ1-29 specifically recognizes CD339, otherwise known as Jagged1, one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.

In humans, mutations in the JAG1 gene are responsible for the autosomal dominant multisystem disorder Alagille syndrome 1 (ALGS1)

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Jagged1-expressing CHO cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

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Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

References for CD339 antibody

  1. Moriyama Y et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
    Int Immunol. 20 (6): 763-73.
  2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
    Arthritis Res Ther. 14: R45.
  4. Koyanagi, A. et al. (2012) Expression of Notch receptors and ligands on immature and mature T cells.
    Biochem Biophys Res Commun. 418: 799-805.

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  2. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  3. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29 (5): 691-703.
  4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol Rev. 182: 215-27.

Synonyms
JAGGED1
RRID
AB_10709279
UniProt
Q9QXX0
Q63722
Entrez Gene
Jag1
Jag1
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0001709 cell fate determination
GO:0016021 integral to membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0042127 regulation of cell proliferation
GO:0042491 auditory receptor cell differentiation
GO:0045596 negative regulation of cell differentiation
GO:0002011 morphogenesis of an epithelial sheet
GO:0009887 organ morphogenesis
GO:0045639 positive regulation of myeloid cell differentiation
GO:0045747 positive regulation of Notch signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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