CD314 antibody | CX5

100% Secure

Rat anti Mouse CD314

Rat anti Mouse CD314:RPE

Product Type
Monoclonal Antibody
Clone
CX5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6056GA F 0.1 mg
MCA6056PE F 100 Tests/0.5ml
Rat anti Mouse CD314 recognizes CD314 also known NKG2D. CD314 is a memeber of the CD92/NKG2 family of C-type lectin receptors. Members of the CD94/NKG2 family are primarily expressed on natural killer or cytotoxic T cells where they activate or inhibit the cytotoxic activities of these cells. CD314 is an activating receptor and functions in collaboration with DAP10. CD314 binds HLA/MHC molecules which are often upregulated under inflammatory conditions.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse CD314
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch

More Information

UniProt
O54709 Related reagents
Entrez Gene
Klrk1 Related reagents
GO Terms
GO:0002223 stimulatory C-type lectin receptor signaling pathway
GO:0016021 integral to membrane
GO:0032394 MHC class Ib receptor activity
GO:0005529 sugar binding
GO:0050830 defense response to Gram-positive bacterium
GO:0009897 external side of plasma membrane
GO:0030101 natural killer cell activation
GO:0032729 positive regulation of interferon-gamma production
GO:0042288 MHC class I protein binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
GO:0071222 cellular response to lipopolysaccharide
Regulatory
For research purposes only

Applications of CD314 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Flow Cytometry 1/8 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA1211 1 ml C E F