CD3 antibody | KT3

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.25 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.1 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 25 µg loader
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Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).

NB. For optimal staining incubations should be performed at room temperature.

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
CBAT6 thymocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized SD rats were fused with cells of the NS0 mouse myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
    Immunogenetics. 28 (6): 445-51.

References for CD3 antibody

  1. Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
    Immunogenetics. 28 (6): 455-8.
  2. Lazarovits, A.I. et al. (1999) Mechanisms of induction of renal allograft tolerance in CD45RB-treated mice.
    Kidney Int. 55: 1303-10.
  3. Heitmann, S. et al. (1999) Immunohistological characterization of leukocytes in the lungs of healthy mice and after bacterial intratracheal infection.
    Lab Anim. 33: 288-94.
  4. Tarlton, J.F. et al. (2000) The role of up-regulated serine proteases and matrix metalloproteinases in the pathogenesis of a murine model of colitis.
    Am J Pathol. 157: 1927-35.
  5. Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
    Infect Immun. 70 (4): 2090-9.
  6. Kumar, L. et al. (2002) Differential role of SLP-76 domains in T cell development and function.
    Proc Natl Acad Sci U S A. 99: 884-9.
  7. Hare, K.J. et al. (2003) Modeling TCR signaling complex formation in positive selection.
    J Immunol. 171: 2825-31.
  8. Erlandsson, L. et al. (2004) Impaired B-1 and B-2 B cell development and atypical splenic B cell structures in IL-7 receptor-deficient mice.
    Eur J Immunol. 34: 3595-603.
  9. View The Latest Product References
  10. Hirsh, M. et al. (2004) Response of lung gammadelta T cells to experimental sepsis in mice.
    Immunology. 112: 153-60.
  11. Severinová, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
    Folia Microbiol (Praha). 50: 457-63.
  12. Bauer, D. et al. (2009) Amniotic membrane transplantation induces apoptosis in T lymphocytes in murine corneas with experimental herpetic stromal keratitis
    Invest Ophthalmol Vis Sci. 50: 3188-98.
  13. Haroon, F. et al., (2011) Gp130-dependent astrocytic survival is critical for the control of autoimmune central nervous system inflammation.
    J Immunol. 186: 6521-31.
  14. Rothhammer, V. et al. (2011) Th17 lymphocytes traffic to the central nervous system independently of α4 integrin expression during EAE.
    J Exp Med. 208 (12): 2465-76.
  15. Salem, M. et al. (2011) Interferon regulatory factor-7 modulates experimental autoimmune encephalomyelitis in mice.
    J Neuroinflammation. 8: 181.
  16. Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
    Acta Neuropathol. 124 (4): 479-90.
  17. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
    EMBO Mol Med. pii: e201404170.
  18. Scheinert, R.B. et al. (2016) Therapeutic effects of stress-programmed lymphocytes transferred to chronically stressed mice.
    Prog Neuropsychopharmacol Biol Psychiatry. 70: 1-7.
  19. Janssen, E. et al. (2016) A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton.
    J Clin Invest. 126 (10): 3837-51.
  20. Van Aelst, L.N. et al. (2016) RNA Profiling in Human and Murine Transplanted Hearts: Identification and Validation of Therapeutic Targets for Acute Cardiac and Renal Allograft Rejection.
    Am J Transplant. 16 (1): 99-110.
  21. Kim, I. et al. (2016) Immunological characterization of de novo and recall alloantibody suppression by CTLA4Ig in a mouse model of allosensitization.
    Transpl Immunol. 38: 84-92.
  22. Massa, M.G. et al. (2017) Testosterone Differentially Affects T Cells and Neurons in Murine and Human Models of Neuroinflammation and Neurodegeneration.
    Am J Pathol. 187 (7): 1613-22.
  23. Granadillo, M. et al. (2019) Impact on antitumor response using a new adjuvant preparation as a component of a human papillomavirus type 16 therapeutic vaccine candidate.
    Vaccine. 37 (30): 3957-60.
  24. Yun, M. et al. (2020) Enriched-Baicalein Attenuates Allergy in Cells and Mice
    Ev-Based Comp Alt Med.. 2020: 1-8.
  25. Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
    J Neuroinflammation. 17 (1): 283.

Flow Cytometry

Functional Assays


Immunohistology - Frozen

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006461 protein complex assembly
GO:0007166 cell surface receptor linked signaling pathway
GO:0042105 alpha-beta T cell receptor complex
GO:0045059 positive thymic T cell selection
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0046982 protein heterodimerization activity
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway
GO:0005515 protein binding


156508 162890 1803




0714 153578

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