CD3 antibody | 145-2C11
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|Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.
CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.
Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.
- Target Species
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488||MCA2356A488||F||100 Tests/1ml||Log in|
|List Price||Your Price|
|Description||Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488|
References for CD3 antibody
Tilley, S.L. et al. (2007) Retinoid-related orphan receptor γ controls immunoglobulin production and Th1/Th2 cytokine balance in the adaptive immune response to allergen.
J Immunol. 178 (5): 3208-18.
Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
Proc Natl Acad Sci U S A. 84 (5): 1374-8.
Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
J Immunol. 146 (8): 2536-43.
Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
J Immunol. 153 (11): 5176-82.
Podd BS et al. (2006) T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice.
J Immunol. 176 (11): 6532-42.
McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
Am J Pathol. 177 (4): 1823-33.
Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
PLoS Med. 5: e239.
Klemann, C. et al. (2015) Interleukin-17, Produced by γδ-T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and is Increased in Livers of Patients.
Gastroenterology. pii: S0016-5085(15)01352-9.
View The Latest Product References
Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
Oncogene. 35 (49): 6341-9.
Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
J Neuroinflammation. 14 (1): 18.
Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
PLoS One. 12 (7): e0181326.
Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
Pharmacol Res. 102: 298-307.
Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
Cardiovasc Res. 114 (3): 456-67.
Ohmura, Y. et al. (2021) Natural Killer T Cells Are Involved in Atherosclerotic Plaque Instability in Apolipoprotein-E Knockout Mice.
Int J Mol Sci. 22(22):12451.
- Entrez Gene
- GO Terms
- GO:0001772 immunological synapse
- GO:0002669 positive regulation of T cell anergy
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0009897 external side of plasma membrane
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032753 positive regulation of interleukin-4 production
- GO:0042105 alpha-beta T cell receptor complex
- View More GO Terms
- GO:0045060 negative thymic T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045879 negative regulation of smoothened signaling pathway
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050852 T cell receptor signaling pathway
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