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CD283 antibody | 11F8

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Anti Mouse CD283 Antibody, clone 11F8 thumbnail image 1 Anti Mouse CD283 Antibody, clone 11F8 thumbnail image 2
Anti Mouse CD283 Antibody, clone 11F8 gallery image 1
Mouse spleen cells stained with Rat anti Mouse CD8 alpha:APC (MCA609APC) and (A) Rat anti Mouse CD283 (MCA5891GA) or (B) rat IgG control. Detection with Goat anti Rat IgG:Dylight®488 was performed following permeabilization of the cells with Leucoperm (BUF09) prior to incubation with Rat anti Mouse CD283 (MCA5891GA).
Anti Mouse CD283 Antibody, clone 11F8 gallery image 2
Mouse spleen cells stained with Rat anti Mouse CD8 alpha:APC (MCA609APC) and (A) Rat anti Mouse CD283:FITC (MCA5891F) or (B) rat IgG control. Staining was performed following permeabilization of the cells with Leucoperm (BUF09A) and incubation with Mouse Seroblock FCR (BUF041).

Rat anti Mouse CD283:FITC

Product Type
Monoclonal Antibody
Clone
11F8
Isotype
IgG2a
Specificity
CD283

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Product Code Applications Pack Size List Price Your Price Qty
MCA5891F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse CD283 antibody, clone 11F8 recognizes mouse TLR3, otherwise known as CD283 or Toll-like receptor 3. CD283 is a 905 amino acid ~105 kDa single pass type 1 transmembrane glycoprotein associated with endosomal membranes and member of the Toll-like receptor (TLR) family. CD283 functions as a receptor for double-stranded RNA (UniProt: Q99MB1).
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. TLRs also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified murine TLR3 extracellular domain protein.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
>
List Price Your Price
loader
Description Rat IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Leucoperm BUF09 F 50 Tests
List Price Your Price
Description Leucoperm

References for CD283 antibody

  1. McCartney, S. et al. (2009) Distinct and complementary functions of MDA5 and TLR3 in poly(I:C)-mediated activation of mouse NK cells.
    J Exp Med. 206: 2967-76.
  2. Jelinek, I. et al. (2011) TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection.
    J Immunol. 186: 2422-9.
  3. McCartney, S.A. et al. (2011) RNA sensor–induced type I IFN prevents diabetes caused by a β cell–tropic virus in mice
    J Clin Invest. 121: 1497-507.
  4. Azuma, M. et al. (2012) Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8α(+) dendritic cells.
    Oncoimmunology. 1: 581-592.
  5. Zeng, R. et al. (2016) Generation and transcriptional programming of intestinal dendritic cells: essential role of retinoic acid.
    Mucosal Immunol. 9 (1): 183-93.
  6. Njoroge, J.M. et al. (2018) A flow cytometric analysis of macrophage- nanoparticle interactions in vitro: induction of altered Toll-like receptor expression.
    Int J Nanomedicine. 13: 8365-78.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Synonyms
TLR3
UniProt
Q99MB1
Entrez Gene
Tlr3
GO Terms
GO:0005515 protein binding
GO:0003725 double-stranded RNA binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0010008 endosome membrane
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034346 positive regulation of type III interferon production
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043330 response to exogenous dsRNA
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0046330 positive regulation of JNK cascade
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus

MCA5891F

1011R 158806

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