CD282 antibody | 6C2

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Rat anti Mouse CD282

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.25 mg loader

Rat anti Mouse CD282 antibody, clone 6C2 recognizes the murine TLR2, otherwise known as CD282, a cell surface molecule that functions as a key receptor in the innate immune response. CD282 may interact functionally at the cell surface with TLR6.

Ligands for CD282 include bacterial cell wall components such as lipoproteins and peptidoglycans.

Product Details

Target Species
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
CHO cells expressing murine TLR2.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0002238 response to molecule of fungal origin
GO:0002687 positive regulation of leukocyte migration
GO:0002752 cell surface pattern recognition receptor signaling pathway
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006954 inflammatory response
GO:0050830 defense response to Gram-positive bacterium
GO:0009897 external side of plasma membrane
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030670 phagocytic vesicle membrane
GO:0032695 negative regulation of interleukin-12 production
GO:0032700 negative regulation of interleukin-17 production
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0035355 Toll-like receptor 2-Toll-like receptor 6 protein complex
GO:0042497 triacyl lipopeptide binding
GO:0042498 diacyl lipopeptide binding
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0042892 chloramphenicol transport
GO:0045087 innate immune response
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046982 protein heterodimerization activity
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0052063 induction by symbiont of defense-related host nitric oxide production
GO:0060907 positive regulation of macrophage cytokine production
GO:0070891 lipoteichoic acid binding
GO:0071223 cellular response to lipoteichoic acid
GO:0071224 cellular response to peptidoglycan
For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

References for CD282 antibody

  1. Kratzer, R. et al. (2010) Fusion proteins for versatile antigen targeting to cell surface receptors reveal differential capacity to prime immune responses.
    J Immunol. 184 (12): 6855-64.
  2. Patole, P.S. et al. (2006) Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice.
    Nephrol Dial Transplant. 21 (11): 3062-73.
  3. Mayer-Barber, K.D. et al. (2011) Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection.
    Immunity. 35 (6): 1023-34.

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