CD28 antibody | 37.51.1

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Hamster anti Mouse CD28

Product Type
Monoclonal Antibody
Clone
37.51.1
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1363 F IP 0.2 mg
Hamster anti Mouse CD28 antibody, clone 37.51.1 recognizes murine CD28, a ~80kDa cell surface glycoprotein that is expressed by most thymocyte subsets and by peripheral T-cells. Studies show that CD28 expression on thymocytes and splenic T-cells can be upregulated upon activation.
Murine CD28 acts as a co-stimulatory signal and may play an important role in regulating the immune response of T-cells.
Hamster anti Mouse CD28 antibody, clone 37.51.1 has been reported to enhance cytokine production and T-cell proliferation in combination with TCR-mediated signals (Gross et al. 1992).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Borate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse T-Lymphoma EL-4 cells.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Fusion Partners
Spleen cells from immunised Syrian Hamsters were fused with cells of the mouse P3XAg8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P31041 Related reagents
Entrez Gene
Cd28 Related reagents
GO Terms
GO:0002863 positive regulation of inflammatory response to antigenic stimulus
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045840 positive regulation of mitosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory
For research purposes only

Applications of CD28 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry NEAT
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR BUF041A/BUF041B.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Product Specific References

References for CD28 antibody

  1. Kawasaki, H. et al. (1999) Increased c-Fos/activator protein-1 confers resistance against anergy induction on antigen-specific T cell.
    Int Immunol. 11 (12): 1873-80.
  2. Gross, J.A. et al. (1992) Identification and distribution of the costimulatory receptor CD28 in the mouse.
    J Immunol. 149 (2): 380-8.