Hamster anti Mouse CD28 antibody, clone 37.51.1
recognizes murine CD28, a ~80kDa cell surface glycoprotein that is expressed by most thymocyte subsets and by peripheral T-cells. Studies show that CD28 expression on thymocytes and splenic T-cells can be upregulated upon activation.
Murine CD28 acts as a co-stimulatory signal and may play an important role in regulating the immune response of T-cells.
Hamster anti Mouse CD28 antibody, clone 37.51.1 has been reported to enhance cytokine production and T-cell proliferation in combination with TCR-mediated signals (Gross et al. 1992
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Mouse T-Lymphoma EL-4 cells.
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Fusion Partners
- Spleen cells from immunised Syrian Hamsters were fused with cells of the mouse P3XAg8.653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
positive regulation of inflammatory response to antigenic stimulus
integral to membrane
induction of apoptosis by extracellular signals
external side of plasma membrane
T cell costimulation
negative thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
positive regulation of mitosis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell proliferation
positive regulation of isotype switching to IgG isotypes
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD28 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR BUF041A/BUF041B.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD28 antibody
Kawasaki, H. et al. (1999) Increased c-Fos/activator protein-1 confers resistance against anergy induction on antigen-specific T cell.
Int Immunol. 11 (12): 1873-80.
Gross, J.A. et al. (1992) Identification and distribution of the costimulatory receptor CD28 in the mouse.
J Immunol. 149 (2): 380-8.