CD279 antibody | RMP1-30
Rat anti Mouse CD279 antibody, clone RMP1-30 (MCA2464) used to detect PD-1 (CD279) expression in mouse cells by flow cytometry.
Image caption:
Confirmation of PD-1 expression as a marker of CD8+CD122+ regulatory T-cells in the CNS of PLPmut mice. (A) Representative example of flow cytometric analysis of T-lymphocytes freshly isolated from brains of untreated PLPmut mice gated for CD8 and CD122 expression. The majority of the CNS-derived CD8+ T-cells are CD122- effector cells (red); only few cells are double-positive for CD8 and CD122 (green) and thus regulatory T-cells. (B) Representative example of flow cytometric analysis of gated CD8+CD122- (red) and CD8+CD122+ (green) T-lymphocytes from brains of untreated PLPmut mice analysed for PD-1-coexpression. Only CD8+CD122+ regulatory cells coexpress PD-1. Right histograms display corresponding counts for CD8+CD122- (red) and CD8+CD122+ cells (green) and their coexpression of PD-1. Appropriate isotype control counts are represented in grey
From: Groh J, Hörner M, Martini R.
Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
J Neuroinflammation. 2018 Jul 3;15(1):194.
doi: 10.1186/s12974-018-1228-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD279
- Product Type
- Monoclonal Antibody
- Clone
- RMP1-30
- Isotype
- IgG2b
- Specificity
- CD279
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD279 antibody, clone RMP1-30 recognizes mouse CD279, a ~55 kDa cell surface protein, a member of the CD28/CTLA-4 family, otherwise known as Programmed Death-1 (PD-1). CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes. CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease (Ansari et al. 2003). Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279. Rat anti Mouse CD279 antibody, clone RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig fusion proteins to PD-1 transfected BHK cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- PD-1 transferred BHK cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD279 antibody
-
Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
J Immunol. 172 (4): 2530-41. -
Bartkowiak, T. (2013) Novel Imaging-Based Techniques Reveal a Role for PD-1/PD-L1 in Tumor Immune Surveillance in the Lung.
UT GSBS Dissertations and Theses (Open Access). Paper 354. -
Hu, Z. et al. (2013) Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect.
J Immunol. 191 (1): 312-22. -
Groh, J. et al. (2018) Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations.
J Neuroinflammation. 15 (1): 194.
- Synonyms
- PD-1
- RRID
- AB_961509
- UniProt
- Q02242
- Entrez Gene
- Pdcd1
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
MCA2464
150124
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